8-甲氧沙林对黑素细胞内游离钙浓度及细胞骨架蛋白形成的影响  

Effects of 8-Methoxypsoralen on intracellular [Ca^(2+)]i and cytoskeleton actin organization in human melanocytes in vitro

作　　者：张宪旗[1] 郑敏[1] 牟宽厚[2] 冯捷[3] 

机构地区：[1]浙江大学医学院附属第二医院皮肤科,浙江杭州310009 [2]西安交通大学第一医院皮肤科,陕西西安710004 [3]西安交通大学第二医院皮肤科,陕西西安710061

出　　处：《浙江大学学报（医学版）》2009年第4期348-351,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省卫生厅科研基金资助课题(2006B057)

摘　　要：目的:观察8-甲氧沙林对体外培养黑素细胞内游离钙浓度、细胞骨架蛋白形成的影响。方法:正常人黑素细胞取自包皮环切术切取的包皮,用激光共聚焦扫描显微镜检测细胞内游离钙浓度,用罗丹明结合毒伞素对细胞骨架蛋白进行特异性染色。结果:8-甲氧沙林可使细胞内游离钙离子浓度升高,促进黑素细胞骨架蛋白合成。结论:8-甲氧沙林可能通过诱导黑素细胞内钙离子浓度升高和细胞骨架actin蛋白生成,从而影响黑素细胞的迁移。Objective： To investigate the effects of 8-methoxypsoralen on human melanocytes [Ca2＋]i and cytoskeleton actin organization in vitro. Methods： Human melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca2＋]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin. Results： 8 -methoxypsoralen increased [Ca2＋ ]i and induced organization of actin stress fiber cytoskeleton. Conclusion： 8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca2＋ concentration and cytoskeleton actin reorganization.

关 键 词：黑素细胞/药物作用 甲氧西林/药理学 钙/代谢 细胞骨架 细胞支架蛋白质类/代谢 细胞 培养的 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]