用于莱茵衣藻荧光定量PCR分析的内参基因选择  

The Selection of Reference Genes in Chlamydomonas reinhardtii P.A. Dangeard by Real-Time Quantitative PCR

作　　者：吴文凯[1,2] 刘成前[2] 周志刚[1] 卢山[2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]南京大学生命科学学院,南京210093

出　　处：《植物生理学通讯》2009年第7期667-672,共6页Plant Physiology Communications

基　　金：国家自然科学基金(30771167);上海市教育委员会重点科研项目(科06-411)

摘　　要：本文用GeNorm与NormFinder算法分析actin、α-tubulin、cblp和yptc1四个常用内参基因在不同处理下莱茵衣藻中的表达。结果显示,α-tubulin在各种条件下都有较好的表达稳定性,而以不同基因组合构成的多内参系统可能更有利于细胞周期短暂的单细胞绿藻的分析。The selection of reference genes plays a key role in the study of gene expression by real-time quantitative PCR. Using GeNorm and NormFinder algorithms, we studied the expression of four common-used reference genes, actin, a-tubulin, cblp and yptcl, in Chlamydomonas reinhardtii under different treatments. Our results showed that the expression of a-tubulin was relatively stable, but it might be more reliable to use a combination of different genes and utilize their normalization factor （NF） as a reference for analyzing the dynamic changing gene expression of unicellular green algae.

关 键 词：莱茵衣藻 荧光定量PCR 内参基因 

分 类 号：Q943[生物学—植物学]