敌枯双和环磷酰胺对SD大鼠胚胎致畸作用比较  

Embryo Malformation Effect Comparison of Bis-A-TDA and Cyclophosphamide

作　　者：胡文娟[1,2] 王玖玖[2] 杨荣富[2] 潘琦[2] 桂博[2] 闫晗[1] 刘向云[2] 吴建辉[2] 周莉[2] 孙祖越[2] 

机构地区：[1]复旦大学药学院,上海200032 [2]上海市计划生育科学研究所药理毒理研究室,中国生育调节药物毒理检测中心,上海200032

出　　处：《生殖与避孕》2009年第7期426-431,共6页Reproduction and Contraception

摘　　要：目的:寻找作为SD大鼠胚胎-胎仔发育毒性试验(Ⅱ段生殖毒性)较理想的阳性对照药物。方法:在大鼠胚胎-胎仔发育毒性试验中,分别在妊娠第10日给予不同剂量敌枯双和环磷酰胺,于妊娠第20日解剖观察胎仔畸形,从致畸种类、致畸率等指标分析比较致畸作用。结果:敌枯双11.0mg/kg组和13.8mg/kg组外观畸形率分别为84.62%和86.84%(P<0.01),而且种类较多,如:脊柱裂、心脏外露、尾畸形、肛门闭锁、骨骼畸形等;环磷酰胺组发生的畸形较少,以骨骼发育迟缓为主。结论:敌枯双对大鼠的致畸率高、致畸种类多,是较好的阳性对照药物。Objective： To look for better positive control drug for embryo-fetal development toxicity test of SD rats. Methods： In the morning of day 10 of gestation the Bis-A-TDA experimental group was administered with Bis-A-TDA 11.0 mg/kg and 13.8 mg/kg, the cyclophosphamide （CP） experimental group was injected with CP 7.2 mg/kg and 9.0 mg/kg to observe malformation of foetus, incidence of malformation on the day 20 of gestation. Results： The incidence of malformation of Bis-A-TDA experimental group was higher up to 84.62% and 86.84% （P〈0.01）, with various kinds, e.g.spina bifida, heart revealed, tail malformation, anal atresia, deformity,etc. Less malformation was appeared in CP experimental group, skeletal hypoevolutism was the mainly malformation. Conclusion： Compared with cyclophosphamide, N, N＇-methylene-bis-（α-amino-1,3,4-thiadiazole） （Bis-A-TDA） is an excellent positive control drug on SD rats which posses high malformation sensitivity.

关 键 词：大鼠 敌枯双(Bis-A-TDA) 环磷酰胺(CP) 致畸试验 脊柱裂 

分 类 号：R992[医药卫生—毒理学]