太空诱变哈密瓜两性花性状连锁标记的RAPD分析  

Identification of RAPD Marker Linked to Hermaphrodite Type in Hami Melon after Space Mutation

作　　者：丁群英[1] 王涛[1] 张瑞 廖新福 郭霭光[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]新疆维吾尔自治区葡萄瓜果开发研究中心,新疆鄯善838201

出　　处：《西北植物学报》2009年第7期1309-1313,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：新疆维吾尔自治区自然科学基金(200721106)

摘　　要：以哈密瓜品种‘早皇后’、太空诱变后两性花株突变体及其后代分离群体为试材,采用BSA方法对哈密瓜两性花性状进行了RAPD分析。研究结果表明:在所筛选的490条10-mer随机引物中,只有S1254在两性花株基因池中扩增到750 bp的多态性条带,而在其它基因型的群体中未扩增到此条带。通过对分离群体及其姐妹系进行单株验证,均获得相同的扩增结果,说明S1254750与两性花性状连锁,该标记与两性花性状的遗传距离为4.5 cM。In the present study, Hami melon ＇Zaohuanghou＇ ,hermaphrodite mutant after space mutation and its offspring group were investigated. Bulked segregation analysis （BSA） was employed to identify randomly amplified polymorphic DNA （RAPD） markers linked to the hermaphrodite type in Hami melon. The results of the study revealed that a 750 bp polymorphic band was amplified with primer S1254 in the hermaphrodite pool in a total of 490 primers screened,while this polymorphic band were not amplified in other genotype groups. The result was confirmed by the validation of the separation groups and sister groups, RAPD marker S1254 750 was proved to be linked to hermaphrodite type,the genetic distance is 4.5 cM.

关 键 词：太空诱变 哈密瓜 两性花性状 RAPD 

分 类 号：Q789[生物学—分子生物学]