症状性颈动脉狭窄患者C反应蛋白＋1444C／T基因多态性分析  

Study on the association of the CRP gene ＋1444C/T polymorphism with symptomatic carotid artery stenosis

作　　者：刘志忠[1] 丁秀荣[2] 郑华光[1] 张果[1] 王瑞珉[1] 康熙雄[1] 

机构地区：[1]首都医科大学附属北京天坛医院检验科,北京100050 [2]华北煤炭医学院附属医院检验科

出　　处：《中华医学遗传学杂志》2009年第4期435-438,共4页Chinese Journal of Medical Genetics

基　　金：“十一五”863重点项目基金（2006AA020706）

摘　　要：目的研究C反应蛋白（Creactive protein，CRP）＋1444C／T基因多态性与症状性颈动脉狭窄的关联。方法应用聚合酶链反应限制性片段长度多态性的方法检测192例症状性颈动脉狭窄患者和197名对照的CRP＋1444C／T基因多态性，同时测定血清高敏C反应蛋白（high sensitivity C—reactive protein，hs—CRP）浓度。结果所有研究对象中未发现TT基因型。患者与对照组、单侧与双侧狭窄组比较，CT基因型频率差异无统计学意义（P〉0．05），狭窄率〉70％与〈70％比较，CC基因型发生〉70％狭窄的风险增加（OR：2．958；95％CI：1.198～7．305；P=0．019）。患者组hs—CRP浓度显著高于对照组，但不同狭窄数目、不同狭窄率比较，hs—CRP浓度差异无统计学意义。结论CRP＋1444C／T多态性是颈动脉〉70％狭窄的遗传风险斟子。CRP浓度升高与颈动脉狭窄发生有关，与狭窄数目、狭窄率无关。Objective To investigate the potential association of the C-reactive protein （CRP） gene ＋ 1444C/T polymorphism with symptomatic carotid artery stenosis. Methods Polymerase chain reaction- restriction fragment length polymorphism was used for the detection of CRP ＋ 1444C/T genotypes in 192 patients with symptomatic carotid artery stenosis and 197 healthy controls. Serum high sensitivity-CRP（hs- CRP） levels were measured by routine method. Results No TT genotype was detected in this study. Patients with 〉 70% stenosis had higher CC genotype compared with those with 〈70% stenosis after adjusting for major cerebrovascular risk factors （OR： 2. 958 ; 95% CI： 1. 198 - 7. 305 ; P = 0. 019）. CRP levels were significantly higher in patients than in controls. Subgroup analysis according to clinical characteristics （single or double stenosis; 〉70%or 〈70% stenosis） did not show difference in CRP levels. There was no significant difference in the prevalence of CT genotype between patients and controls, or between single and double stenosis（P〉 0.05）. Conclusion The CRP ＋1444 CC genotype is a risk factor for 〉70% carotid artery stenosis. The serum CRP level is associated with the presence of carotid stenosis. However, it is not associated with the number and severity of stenosis.

关 键 词：颈动脉狭窄 动脉粥样硬化 C-反应蛋白 遗传多态性 

分 类 号：R686[医药卫生—骨科学]