Expression of the Arabidopsis thaliana Histone Gene AtHTA1 Enhances Rice Transformation Efficiency  

作　　者：Ye Zheng Xiao-Wei He Ying-Hui Ying Jiang-Feng Lu Stanton B. Gelvin Hui-Xia Shou 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Yuhangtanghe Road 368, Hangzhou 310058, China [2]Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA

出　　处：《Molecular Plant》2009年第4期832-837,共6页分子植物（英文版）

摘　　要：We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA 1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA 1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA 1 either transiently or in stably transformed cells improved rice transformation efficiency.We expressed the Arabidopsis thaliana histone AtHTA1 in rice under the control of the maize ubiquitin promoter. Transformation efficiencies of rice plants that constitutively expressed AtHTA 1 were 28-44% higher than calli containing an empty vector control. Furthermore, co-infection of rice calli with a vector containing AtHTA 1 and another vector with the target gene increased transformation by 27-50%. Thus, expression of AtHTA 1 either transiently or in stably transformed cells improved rice transformation efficiency.

关 键 词：ARABIDOPSIS histone H2A AGROBACTERIUM RICE transformation. 

分 类 号：Q943[生物学—植物学] S511.01[农业科学—作物学]