流式细胞术检测慢性淋巴细胞性甲状腺炎患者外周血Th1、Th2细胞的意义  

Significance of the T helper 1 and T helper 2 lymphocytes in peripheral blood of patients with chronic lymphocytic thyroiditis as detected by flow cytometry

作　　者：王云云[1] 宋进展[1] 吴汉妮[1] 钱伟[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,武汉430022 [2]华中科技大学同济医学院附属协和医院胃肠病研究室

出　　处：《现代免疫学》2009年第4期320-323,共4页Current Immunology

摘　　要：检测慢性淋巴细胞甲状腺炎(CLT)患者外周血Th1、Th2细胞的水平,探讨Th1细胞占CD4^+T细胞比例(Th1%)、Th2细胞占CD4^+T细胞比例(Th2%)、Th1/Th2在各组间的差异和意义及分别与血清中针对甲状腺特异抗体的相关性。收集45例CLT患者外周血,分为甲状腺功能正常组15例和甲状腺功能减退组30例,20例健康人外周血作为对照组;采用胞内细胞因子染色流式细胞术检测Th1%和Th2%;应用电化学发光法检测甲状腺自身抗体——甲状腺球蛋白抗体(TgAb)和甲状腺过氧化物酶抗体(TPOAb)水平。结果显示,两组CLT患者外周血Th1%、Th1/Th2均高于正常对照组,差异均有统计学意义(P<0.05),CLT两组间Th1%、Th1/Th2的差异无统计学意义;各组间Th2%差异无统计学意义;CLT患者外周血Th1%、Th1/Th2分别与血清TPOAb及TgAb阳性表达呈显著正相关(P均<0.01)。进一步证实Th1细胞在CLT发病中起主导作用,Th1/Th2细胞失衡且向Th1细胞漂移与CLT发生发展密切相关,并且提示胞内细胞因子染色流式细胞术检测Th1/Th2细胞可成为研究自身免疫性甲状腺炎免疫状态的可靠方法。This study was designed to detect the ratio of Thl lymphocytes （Th1%） and the Th2 lymphocytes subpopulations （Th2%） in peripheral blood of patients with chronic lymphocytic thyroiditis（CTL）, and to investigate the difference and significance of Th1% and Th2%. In addition, the difference and significance of Th1/Th2 radio among different groups and the correlations between the positive expression of serum thyroid autoantibody were also determined. In the present study, peripheral blood samples were collected from 45 patients with CTL including 15 cases with normal thyroid function, and 30 cases with hyothyroidism and those taken from 20 healthy individuals were used as controls Th1% and Th2% were evaluated by flow cytometry, and the level of serum thyroglohulin antibodies（TgAb） and thyroid peroxidase antibodies（TPOAb） were assessed with electrochemiluminescence assay. It was demonstrated that both Th15 and Thl/Th2 were significantly higher in patients with CLT than the healthy control group （P〈0.05）, While those of Thl/Th2 had no significant difference between CLT pa tients with normal thyroid function and CLT patients with hypothyroidism（P〉0.05）, and the difference in Th2% showed no significant change （P〉0. 05）. There were significant correlations between the positive expression of serum thyroid autoantibody（TPOAb, TgAh） and Th1% as well as the Th1/Th2 ratio. It is concluded that the increase of Th1% and the imbalance of Th1/Th2 may play an important role in the occurrence and process of CLT, and that the detection of Th1/Th2 cells by flow cytometry can be recognized as a reliable way to study the immune state of autoimmune thyroiditis.

关 键 词：慢性淋巴细胞甲状腺炎 TH1细胞 TH2细胞 流式细胞术 

分 类 号：R392.6[医药卫生—免疫学]