Inhibition of P2X receptor-mediated inward current by protein kinase C in small-diameter dorsal root ganglion neurons of adult rats  

蛋白激酶C抑制P2X受体介导的大鼠小直径背根节神经元内向电流(英文)

作　　者：别毕华[1] 张一红[1] 赵志奇[1] 

机构地区：[1]复旦大学神经生物学研究所,脑科学研究院,国家医学神经生物学重点实验室,上海200032

出　　处：《Neuroscience Bulletin》2009年第4期179-186,共8页神经科学通报（英文版）

基　　金：supported by grants from the National Basic Research Development Program of China (No.2006CB500807);the National Natural Science Foundation of China (No.30600178)

摘　　要：Objective The aim of the present study is to verify the ATP-induced varied responses in isolated dorsal root ganglion （DRG） neurons of the adult rat, and investigate the modulatory effects of specific P2X receptor agonist β,γ-me-ATP and protein kinase C （PKC） on P2X receptor-mediated inward current in DRG neurons. Methods Whole cell patch-clamp was employed to record the currents on acutely isolated DRG neurons in the adult rats. Results β,γ-me-ATE similar as ATE evoked 2 distinct subtypes of P2X receptor-mediated inward currents in a dose-dependent manner in DRG neurons. Activation of PKC by phorbol 12,13-dibutyrate （PDBu） significantly inhibited both subtypes of inward currents mediated by P2X receptors in a dose-dependent manner. Conclusion Activation of PKC negatively modulated the P2X receptor-mediated currents in rat DRG neurons, which may be of benefit to preventing the over-excitation of nociceptor under inflammatory or neuropathic conditions.目的在成年大鼠背根节神经元,观察ATP诱导的多样性反应,研究P2X激动剂β,γ-me-ATP以及蛋白激酶C对P2X受体介导的内向电流的调制作用。方法应用全细胞膜片钳记录急性分离的大鼠背根节神经元电活动。结果与ATP诱导的反应相同,P2X激动剂β,γ-me-ATP也引起P2X受体介导的两种类型的内向电流,并呈剂量依赖性。蛋白激酶C激动剂phorbol 12,13-dibutyrate(PDBu)能显著抑制P2X受体介导的两类内向电流,并呈剂量依赖关系。结论蛋白激酶C负向性调制大鼠背根节神经元的P2X受体介导的内向电流,提示蛋白激酶C的激动剂可用于防止炎症和神经病理条件下伤害性感受器的过度兴奋。

关 键 词：dorsal root ganglion P2X ATP β γ-me-ATP protein kinase C 

分 类 号：Q42[生物学—神经生物学]