盐酸林可霉素滴眼液中主药含量的测定方法改进  

Improvement of the Determination Method of Main Component in Lincomycin Hydrochloride Eye Drops

作　　者：方焱[1] 舒冰[1] 张善堂[1] 陈象青[1] 

机构地区：[1]安徽医科大学附属省立医院药剂科,合肥市230001

出　　处：《中国药房》2009年第22期1739-1741,共3页China Pharmacy

摘　　要：目的:改进盐酸林可霉素滴眼液含量测定方法以排除其中抑菌剂羟苯乙酯对主药含量测定的干扰。方法:采用高效液相色谱法,将国家化学药品标准法(A法)中的流动相硼砂缓冲液(pH6.0)-甲醇(4:6)改为磷酸缓冲液(pH6.8)-甲醇(40:60),将2种分别含有和不含有羟苯乙酯的样品含量测定结果与A法进行比较。结果:不含羟苯乙酯样品的测定结果2种方法比较无显著性差异,含有羟苯乙酯样品的测定结果A法远高于标示量及改进方法所测结果。结论:改进方法可用于该制剂的含量测定。OBJECTIVE： To improve the determination method for lincomycin hydrochloride eye drops so as to eliminate the interference of the bacterial inhibitor ethylparaben on the content of the main component. METHODS： The determination was performed by HPLC with phosphoric buffer （pH 6.8） -methanol （40 ： 60） as the mobile phase replacing the mobile phase of borax buffer （pH6.0） -methanol （4 ： 6） recommend in state chemicals standard technique （Method A） . The contents of ethylparaben - contained sample and ethylparaben - free sample determined by HPLC were compared with those determined by Method A. RESULTS： There was no significant difference in the content of ethylparaben free sample between the two determination methods, but the content of ethylparaben- contained sample determined by Method A was higher than both the labeled amount and that determined by the improved method. CONCLUSION： The improved method is applicable for the content determination of lincomycin hydrochloride eye drops.

关 键 词：盐酸林可霉素 滴眼液 高效液相色谱法 改进 

分 类 号：R927.2[医药卫生—药学] R978.1