灯盏花提取物对异基因大鼠肾移植外周血树突状细胞的影响  

Effects of erigeron extract on dendritic cells in rat peripheral blood following allogeneic kidney transplantation

作　　者：王洪波[1] 龙刚[2] 

机构地区：[1]天津医科大学,天津市300070 [2]天津市人民医院,天津市300121

出　　处：《中国组织工程研究与临床康复》2009年第31期6041-6044,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:鉴于树突状细胞迁移在急性排斥反应发生机制中占据重要地位,人们通过使用某些药物延缓树突状细胞的迁移或通过阻断未成熟树突状细胞向成熟树突状细胞的转变来防治急性排斥反应。目的:观察灯盏花提取物对异基因大鼠肾脏移植模型血液中树突状细胞的影响。设计、时间及地点:随机对照动物实验,于2008-11/2009-05在天津市人民医院进行。材料:灯盏花提取物由南开大学化学元素研究所提供。灯盏花属掌叶大黄类植物,提取物中包含2,5-二甲基-7-羟基色原酮、5,7,4’-三羟基黄酮(黄酮甙元)。肾脏供体为32只清洁级雄性Wistar大鼠,32只SD大鼠为受体。方法:行原位左肾移植手术并同时切除受体右侧肾脏,移植后,按照随机数字表法分为生理盐水组、灯盏花提取物低、中、高剂量组。灯盏花提取物低、中、高剂量组分别腹腔注射灯盏花提取物5,10,15mg/(kg?d),生理盐水组腹腔注射生理盐水。术后5d收集大鼠外周血,经红细胞裂解液和淋巴细胞分离液处理后,接种于培养皿中,2h后除去悬浮细胞,调整浓度1×109L-1加入1640完全培养液放于37℃、体积分数5%CO2培养箱中,第3天半量换液,第7天收获细胞。主要观察指标:观察树突状细胞形态并测定成熟状态;酶联免疫吸附法检测白细胞介素12水平;混合淋巴细胞培养测定细胞的抗原递呈功能。结果:生理盐水组树突状细胞树突分支增加、增粗、近圆形,体积较大,多角形或具有特征性的星形。灯盏花提取物作用下的细胞相对幼稚、稀疏,高剂量时差异更明显。灯盏花提取物组CD40、CD80、主要组织容性抗原Ⅱ表达率和白细胞介素12分泌量较生理盐水组明显降低(P<0.01),随灯盏花提取物剂量增加,下降更明显(P<0.01)。反应和刺激细胞按10∶1比例混合后,生理盐水组树突状细胞刺激淋巴细胞增殖的作用最强(P<0.01),随灯盏花提取物剂量增加BACKGROUND： Dendritic cell migration occupies an important place in the mechanism of acute rejection; therefore, acute rejection can be prevented and cured by slowing down the migration of dendritic cells with certain drugs or by blocking mature of dendritic cells. OBJECTIVE： To detect the effect of erigeron extract on dendritic cells in rats＇ blood with allogeneic kidney transplant models. DESIGN, TIME AND SETTING： A randomized controlled animal experiment was carded out in Tianjin Union Medicine Centre from November 2008 to May 2009. MATERIALS： Erigeron extract was supplied by Institute of Elemento-organic Chemistry, Nankai University. Erigeron is Rheum palmatum plants, which extracts contained 2,5-dimethyi-7-hydroxy-chromone, 5,7,4 ＇- trihydroxy-flavone （flavonoid million） with formula of C15H1005. Thirty-two male Wistar rats of clean grade were served as kidney donor, and 32 SD rats as recipients. METHODS： Orthotopic renal transplant was performed at the left kidney, simultaneously, the right kidney was removed. After transplantation, in accordance with the random number table, all rats were divided into the physiological saline, and erigeron extract with low, medium and high dose groups. Extracts erigeron with 5, 10, 15 mg/（kg · d） were injected into erigeron extract group. At 5 days after transplantation, peripheral blood was collected, treated by erythrocyte lysis and lymphocyte separation medium, and then inoculated in a Petri dish. Suspended cells were removed after 2 hours, followed by adjusting the concentration of 1 × 10^9/L by adding 1640 full culture medium, and then put the cell at 37 ℃, 5% CO2 incubator. The liquid was half renewed every 3 days, and the cells were harvested at the 7 days. MAIN OUTCOME MEASURES： The morphology and mature state of dendritic cells were determined; the interleukin-12 （IL-12） level was measured by enzyme-linked immunosorbent assay; in addition, antigen-presenting function was detected by mixed lymphocyte culture （MLR）. RESULTS： In

关 键 词：树突状细胞 大鼠 肾脏移植 白细胞介素12 混合淋巴细胞培养 

分 类 号：R617[医药卫生—外科学]