受体血预先经门静脉输入供体诱导移植肝浸润细胞凋亡(英文)  

Induction of hepatic allograft-infiltrating T lymphocyte apoptosis by pretransplant intraportal infusion of recipient blood into donor

作　　者：王凤山[1] 张佳林[1] 杨蕾[1] 程颖[1] 邵自功[1] 刘永锋[1] 

机构地区：[1]中国医科大学附属第一医院普通外科教研室肝胆外科,辽宁省沈阳市110001

出　　处：《中国组织工程研究与临床康复》2009年第31期6185-6188,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:肝移植后排斥反应发生时,肝细胞被浸润的T淋巴细胞破坏,肝细胞减少,肝功能进行性恶化。诱导肝移植免疫耐受仍然是目前面临的重大课题。目的:观察受体血预先经门静脉输入供体对大鼠移植肝内浸润淋巴细胞凋亡的影响。设计、时间及地点:随机对照动物实验,于2002-05/2004-05在中国医科大学器官移植实验室完成。材料:选用纯系大鼠制作大鼠原位肝移植模型。24只供体为雄性ACI大鼠(RT1a),24只受体为雄性LEW大鼠(RT1l)。方法:大鼠肝移植采用改良的两袖套法进行原位移植。受体大鼠按随机数字表法分为4组,每组6只。对照组未作处理;受体血经阴茎静脉输入组于移植前7d将受体血1mL经阴茎静脉输入供体;非受体血经门静脉输入组于移植前7d将非受体大鼠(BN大鼠)血1mL经门静脉输入供体;受体血经门静脉输入组于移植前7d将受体血1mL经门静脉输入供体。主要观察指标:肝移植后观察大鼠生存时间;移植后检测各组大鼠血清γ-干扰素质量浓度;移植后观察移植肝组织学变化;移植后7d检测移植肝内树突状细胞数量;移植后3,5,7,14d检测移植肝内浸润淋巴细胞的凋亡情况。结果:受体血经门静脉输入组大鼠的生存时间显著长于对照组(P<0.01)。肝移植后3,5d,受体血经门静脉输入组大鼠的血清γ-干扰素质量浓度显著高于对照组(P<0.05),受体血经阴茎静脉输入组、非受体血经门静脉输入组大鼠的生存时间及血清γ-干扰素质量浓度与对照组无显著差异(P>0.05)。受体血经门静脉输入组大鼠移植肝汇管区内的淋巴细胞浸润显著减少,移植肝内检测到大量供体来源的树突状细胞。移植后受体血经门静脉输入组大鼠肝组织切片每平方毫米的凋亡细胞数显著多于对照组(P<0.01)。结论:受体血预先经门静脉输入供体,可以延长异系肝移植受体大鼠生存时间,促进移植肝内浸润淋巴细胞的凋�BACKGROUND： When immunological rejection occurs following liver transplantation, liver cells are destroyed by infiltrated T lymphocytes, leading to progressive deterioration of hepatic function owing to reduction of liver cells. Induction of immunological tolerance of liver transplantation remains a challenge. OBJECTIVE： To observe the influences of pretransplant intraportal infusion of recipient blood into donor on the apoptosis of hepatic allograft-infiltrating T lymphocytes in rats. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Organ Transplant Unit, China University between May 2002 and May 2004. MATERIALS： Inbred rats were developed into models of orthotopic liver transplantation. Twenty-four female ACI rats （RTla） served as donors, and an additional twenty-four male LEW rats （RT11） served as recipients. METHODS： A modification of cuff method was employed for orthotopic liver transplantation in rats. Twenty-four recipient rats were randomly and evenly divided into 4 groups： （1） control： no treatment; （2） penile vein infusion of recipient blood： 1 rnL recipient blood was infused into each donor rat via the penile vein;（3） intraportal infusion of non-recipient blood： 1 mL non-recipient rat （BN） blood was infused into each donor rat via the portal vein; （4） intraportal infusion of recipient blood： 1 mL recipient blood was infused into each donor rat via the portal vein. All blood infusions were performed 7 days prior to liver transplantation. MAIN OUTCOME MEASURES： Rat survival time, serum content of y- interferon, histological changes of hepatic allograft, number of dendritic cells in the hepatic allograft, and T lymphocyte apoptosis following liver transplantation. RESULTS： Rat survival time was significantly longer in the intraportal infusion of non-recipient blood group than in the control group （P 〈 0.01）. At 3 and 5 days after liver transplantation, the intraportal infusion of non-recipient blood group e

关 键 词：肝移植 免疫耐受 凋亡 大鼠 

分 类 号：R617[医药卫生—外科学]