昆虫RNAi技术及其应用  

RNA interference and its application in Entomology

作　　者：何正波[1] 陈斌[1] 冯国忠[1] 

机构地区：[1]重庆师范大学生命科学学院昆虫与分子生物学研究所,重庆400047

出　　处：《昆虫知识》2009年第4期525-532,共8页Entomological Knowledge

基　　金：国家自然科学基金项目(编号:30700435);(编号:30870340);重庆市自然科学基金项目(编号:2007BB1227)

摘　　要：RNAi是近几年发展起来的抑制基因表达的新技术。部分昆虫存在RNAi信号的系统性传播现象,可以将dsRNA直接注射进昆虫的卵、血腔或局部组织,引发远距离靶基因的特异性沉默,建立起了Embryo RNAi,Larval RNAi,Adult RNAi,Parental RNAi,Feeding RNAi和基于转基因技术的可遗传RNAi等昆虫RNAi技术,使RNAi迅速成为了研究昆虫尤其是非模式昆虫基因功能的主要方法。文章拟就RNAi的系统性、昆虫RNAi技术及其应用进行综述。RNAi is a new technology for inhibiting gene expression in diverse organisms in recent years. The RNAi effect could spread from cells to cells in some insects, which induce the targeted gene specifically silenced in other tissues by introducing dsRNA into embryos, cavity or partial tissues. As a result, several methods of RNAi in insects have been reported, such as embryo RNAi, larval RNAi, adult RNAi, parental RNAi, feeding RNAi and heritable RNAi based on transgenic technique. These techniques have become widely used tools to knock down gene expression and analyze gene function, especially in non-model insects which the systematic recovery of mutants is not feasible. This paper focused on the systemic gene silencing of RNAi, RNAi technology and its applications in Entomology.

关 键 词：RNAI 系统性基因沉默 昆虫 应用 

分 类 号：Q963[生物学—昆虫学]