用基因组重排技术选育赖氨酸高产菌株  

Screening and breeding of high lysine-producing strains by genome shuffling

作　　者：赵凯[1] 段巍[1] 孙立新[2] 周东坡[1] 

机构地区：[1]黑龙江大学生命科学学院,微生物黑龙江省高校重点实验室,哈尔滨150080 [2]黑龙江大学校医院,哈尔滨150080

出　　处：《微生物学报》2009年第8期1075-1080,共6页Acta Microbiologica Sinica

基　　金：黑龙江省重大科技攻关项目(GA02C101);黑龙江大学杰出青年科学基金项目资助~~

摘　　要：【目的】以北京棒杆菌(Coryne bacterium pekinense)1为研究对象,选育赖氨酸高产菌株,并探索赖氨酸产生菌基因组重排育种的基本规律。【方法】利用基因组重排技术选育赖氨酸高产菌株。【结果】通过四轮基因组重排成功选育出了5株遗传稳定的高产赖氨酸菌株,其中1株重排菌株赖氨酸产量达到16.95g/dL,比原始菌株Corynebacterium pekinense1赖氨酸产量提高了37.14%,比亲本菌株赖氨酸产量提高了17.46%～31.19%。【结论】首次采用基因组重排技术改良赖氨酸产生菌,成功选育出了5株产量较稳定的高产赖氨酸菌株,具有潜在的应用价值。[Objective] To screen and breed high lysine-producing strains by genome shuffling. [Methods] Corynebacterium pekinense 1 was used as the starting strain in this study. High lysine-producing strains were screened by genome shuffling. [ Results] Five hereditarily stable strains with high lysine production were obtained by four cycles of genome shuffling. A high lysine producing strain, F4-36, was obtained, which produced 16.95 g/dL lysine, 37.1% higher than that of the starting strain. [ Conclusion] Genome shuffling can be an efficient tool to screen and breed high lysine production strains.

关 键 词：赖氨酸 赖氨酸产生菌 基因组重排 

分 类 号：Q78[生物学—分子生物学]