C3d-M28增强伪狂犬病毒gC基因体液免疫  

C3d-M28 enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus

作　　者：樊惠英[1] 刘中勇 佟铁铸[3,4] 刘星 郭爱珍[4] 

机构地区：[1]华南农业大学兽医学院,广州528427 [2]广东省出入境检验检疫局技术中心,广州541004 [3]惠州市出入境检验检疫局技术中心,惠州516006 [4]华中农业大学畜牧兽医学院,武汉430070

出　　处：《生物工程学报》2009年第7期987-992,共6页Chinese Journal of Biotechnology

基　　金：国家自然科学基金项目(No.30800826)资助~~

摘　　要：研究补体C3d的受体结合功能区(M28)对伪狂犬病毒gC基因DNA疫苗免疫增强作用。将4拷贝的M28基因与伪狂犬病毒gC基因串联后,克隆到载体pcDNA3.1中,构建融合表达的重组质粒(sgC-M284)。BALB/c小鼠免疫试验表明,sgC-M284免疫组比单独表达伪狂犬病毒gC蛋白的重组质粒(sgC)免疫组产生的ELISA抗体高17倍,对致死剂量(316LD50)伪狂犬病毒攻毒的保护率提高了63%。gC基因与M28基因融合表达诱导产生的IL-4水平接近了伪狂犬灭活疫苗免疫组的产生的IL-4水平,显著增强了基于Th2途径的体液免疫反应。We studied the immunogenicity of pseudorabies virus gC DNA vaccination by fusing the murine complement C3d receptor binding domain. First, pseudorabies virus gC gene was linked to four copies of C3d receptor binding domain （M284）, and then cloned into the vector pcDNA3.1 to construct the recombinant plasmid sgC-M284. Through the experiment of immunized BALB/c mice, we found that the enzyme linked immunosorbent assay （ELISA） antibody titer for sgC-M284 was 17-fold higher than that for sgC alone, and protective rate of mice was augmented from 25% to 88% after lethal dose PrV （316 LD50） challenge. In addition, the IL-4 levels for sgC-M284 immunization approached that for the pseudorabies virus inactivated vaccine. In conclusion, we demonstrated murine C3d receptor binding domain fusion significantly increased Th2-biased immune response by inducing IL-4 production.

关 键 词：补体C3d gC糖蛋白 伪狂犬病毒 C3d受体结合功能区(M28) 分子免疫佐剂 

分 类 号：S852.65[农业科学—基础兽医学]