地塞米松与参附对大鼠皮瓣缺血再灌注损伤后肿瘤坏死因子α的调节作用  

The regulating role of TNF-α with dexamethasone and shenfu separate and joint administration of the flap after ischemia-reperfusion injury

作　　者：黄卫[1] 赖晓敏[1] 张应鹏[1] 钟波[1] 王素伟[1] 

机构地区：[1]珠海市人民医院脊柱骨病科,广东省珠海519000

出　　处：《中国基层医药》2009年第7期1178-1179,共2页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的探讨地塞米松与参附单独和联合用药对皮瓣缺血再灌注损伤的保护作用和机制。方法40只月龄相当的健康大鼠随机分为A、B、C、D四组，制作腹部岛状皮瓣，分别于阻断血流前30min皮瓣蒂部动脉注射0．9％NaCl注射液、地塞米松1ml／kg、参附注射液10ml／kg、联合注射地塞米松1ml／kg加参附注射液10ml／kg。四组动物均在蒂部血管阻断前1h及再灌注后1h,6h、12h、24h分别从蒂部静脉采集血样，测定血浆肿瘤坏死因子α（TNF—α）含量。结果各治疗组术后各时段TNFα浓度明显低于空白组（P〈0．01），D组与B、C组也有明显差异（P〈0．01）。结论早期使用地塞米松、参附注射液能降低皮瓣再灌注损伤后血TNF-α浓度，对皮瓣具有保护作用。联合使用效果更佳。Objective To discuss the protective effects and mechanisms ofdexamethasone and Shenfu separate and joint administration of flap after ischemia-reperfusion injury. Methods 40-month-old fairly healthy rats were randomly divided into 4 group as A, B, C, D, and to product the abdominal island flap, then blocking the flow of blood of the pedicle artery respectively before 30 minutes when injecting with normal saline （ 1 ml/kg）, dexamethasone （1 ml/kg）, Senate （10 ml/kg）, joint injection with dexamethasone （1 ml/kg） and Shenfu （10 ml/kg）. 4 groups of animals＇ blood samples were collected from the pedicle vein before the time of 1 hour when blocking vascular pedicle and reperfusion after the time of 1 h, 6 h, 12 h, 24 h, respectively. Then the plasma concentration of TNF-α was measured. Results The concentration of TNF-α in the treatment group was significantly lower than the blank group（ P 〈0.01 ）. And the group D and the group B,C has significant differences either（ P 〈 0. 01 ）. Conclusion Using Dexamethasone, Shenfu injection in early can reduce the concentration of TNFα in reperfusion injury of flap and has a protective effect on the flap, but make better effect in combined.

关 键 词：缺血再灌注损伤 肿瘤坏死因子Α 参附注射液 地塞米松 

分 类 号：R6[医药卫生—外科学]