水/有机溶剂体系中产紫青霉全细胞催化合成单葡糖醛酸基甘草次酸  

Biosynthesis of glycyrrhetinic acid monoglucuronide by whole-cell of Penicillium purpurogenum in an aqueous/organic system

作　　者：邹树平[1,2] 张琦[1] 李丽敏[1] 郭燕霞[1] 戴大章[1] 李春[1,2] 

机构地区：[1]北京理工大学生命学院,北京100081 [2]天津大学化工学院,天津300072

出　　处：《化工学报》2009年第8期2040-2045,共6页CIESC Journal

基　　金：国家自然科学基金项目(20776017);北京市自然科学基金项目(5072028);北京理工大学研究生科技创新项目(GA200807);北京理工大学基础研究基金项目(20060642004;20081642001)~~

摘　　要：研究了产紫青霉Penicillium purpurogenum Li-3全细胞β-D-葡萄糖醛酸苷酶在水/有机溶剂体系中催化水解甘草酸,合成单葡糖醛酸基甘草次酸(GAMG)的反应。确定了最适反应体系为水/叔丁醇(体积比为3∶7),最适反应条件为:底物甘草酸用量1.5g·L-1、反应温度45℃、pH值5.0、全细胞酶量7g·L-1、摇床转速120r·min-1,在此条件下反应48h,产物GAMG产率可达79.12%,全细胞酶具有较好的稳定性和可重复使用性,显示了良好的应用前景。Biosynthesis of glycyrrhetinic acid monoglucuron whole cell of Penicillium purpurogenum Li-3 in an aqueous e （GAMG） by β-D-glucuronidase from the organic system was studied. Results showed that tert-butyl alcohol is the best organic solvent and the optimum volume ratio of aqueous to organic phase is 3 to 7. The rest optimum conditions for this biocatalysis are 1.5 g ·L^-1 of substrate concentration, 45℃ of temperature, pH 5.0, 7 g ·L^-1 of enzyme concentration and 120 r · min^-1 of shaking rate. Under these optimized conditions, 79.12% yield of GAMG was achieved after 48 h reaction. The whole-cell enzyme has a good reusability and a high stability, which demonstrates the prospect for real application.

关 键 词：生物转化 单葡糖醛酸基甘草次酸 水/有机溶剂体系 产紫青霉 全细胞 β-D-葡萄糖醛酸苷酶 

分 类 号：Q814.9[生物学—生物工程]