黄连素与头孢菌素联用对产ESBLs克雷伯菌的体外抗菌作用初探  

Study on in vitro antibacterial effect of Berberine on ESBLs-producing K.Pneunmoniae Combing with Cephalothin

作　　者：米伟[1] 张永海[1] 

机构地区：[1]信阳职业技术学院医学检验系,信阳464000

出　　处：《中国医疗前沿》2009年第14期1-2,10,共3页China Healthcare Innovation

摘　　要：目的探讨黄连素与头孢他啶联用对产ESBLs克雷伯菌抑菌作用。方法采用纸片扩散法、琼脂稀释法和液体稀释法测定。结果头孢他啶对产ESBLs克雷伯菌MIC90为19.55μg·ml-1,黄连素对产ESBLs大肠埃希菌MIC90为35.67mg·ml-1;黄连素及黄连素+头孢他啶使β-内酰胺酶活性降低,P<0.05;单用头孢他啶与头孢他啶联合黄连素所测抑菌圈直径均数比较,P<0.05,有统计学意义;头孢他啶、黄连素联合药敏试验对产ESBLs克雷伯菌FIC指数为0.75、0.625、0.625。结论黄连素联合头孢他啶对产ESBLs克雷伯菌联合药敏结果呈相加作用,可抑制产ESBLs克雷伯菌的β-内酰胺酶活性。Object To explore antibacterial effect of Berberine on ESBLs-producing K. Pneunmoniae combing with Ceftazidime. Methods ESBLs-producing K. Pneunmoniae confirmation test was done using the method of slip; MIC of Berberine and Ceftazidime on ESBLs-producing K. Pneunmoniae was detected by agar dilution test and double dilution; Evaluation of synergistic antibacterial effect was used by broth chessboard method. Antimicrobial susceptibilities test was done using the methods of Kirby-Bauer according to the standards of CLSI. Results MIC90 of Ceftazidime on ESBLs-producing K. Pneunmoniae is 19.55μg·ml-1,and MIC90 of Berberine on ESBLs-producing K. Pneunmoniae is 35.67mg·ml-1 ,Activity of β-Lactamase of Berberine is significant lower than Berberine＋Ceftazidime by bacterio-lipid compared with bacterio broth（P〈0.05）;The diameter of antibacterial ring of Ceftazidime on ESBLs producing K. Pneunmoniae is 12-23mm,while that of Berberine＋Ceftazidime is 13-24.Smm,which there is significant difference using t-test（P〈0.05）;FIC index of Berberine＋Ceftazidime is additive action by antimicrobial susceptibilities test of ESBLs- producing K. pneunmoniae. Conclusion Antibacterial effect of Berberine on ESBLs-producing K. Pneunmoniae combing with Ceftazidime is additive action by antimicrobial susceptibilities test; Berberine can inhibit activity of B-Lactamase of ESBLs-producing K. pneunmoniae combing with Ceftazidime.

关 键 词：黄连素 头孢他啶 药敏试验 产ESBLs克雷伯菌 

分 类 号：R378.2[医药卫生—病原生物学]