3组瓶法测定水溶性高聚物的生物降解率  

Determination of biodegradation rates of water-soluble polymers by three-group-bottles method

作　　者：吴新世[1] 刘连遵[1] 孙波[1] 王菁[1] 师慈[1] 

机构地区：[1]天津理工大学生物工程系,天津300191

出　　处：《天津师范大学学报（自然科学版）》2009年第3期58-61,共4页Journal of Tianjin Normal University：Natural Science Edition

摘　　要：以水溶性聚天冬氨酸壳聚糖共聚物为研究对象,初步建立了一套以聚合物降解3组瓶即样品瓶、毒性瓶和中性瓶培养为核心内容,包括样品预处理、检测方法选择、结果分析处理等的水溶性高聚物生物降解率测定的基本方法.与常规分析方法相比,一方面本实验方案因增加了中性瓶设计而使得其逻辑性更加严密;另一方面,将聚天冬氨酸壳聚糖共聚物用活性污泥滤液处理28 d,由本实验方案得到的生物降解率为55.47%,而常规方法仅为47.51%,前者比后者约高出7.96%.A three-group-bottles method namely culturing bottle, toxic bottle, and neutral bottle for determining the biodegradability of water-soluble polyaspartic acid-chitosan copolymer was established and studied from the pretreatment of the remained sample, choice of the analyzing method, and analysis of the testing data, etc. Compared with the routine testing method, the basic determination approach had two advantages at least： on the one hand, it had more logic by working out the neutral bottle in the test design, and on the other, after the copolymer was treated by the active sludge for 28 days simultaneously, the maximum degradation rate arrived to 55.47% by the determination approach, while that was only 47.51% by the latter, which was lower 7.96% than the former.

关 键 词：水溶性高聚物 生物降解率 3组瓶方法 

分 类 号：Q939.9[生物学—微生物学]