Purification and characterization of extracellular chitinase from a novel strain Aspergillus fumigatus CS-01  

作　　者：夏金兰 熊晶 徐涛 张成桂 张瑞永 张倩 武顺 邱冠周 

机构地区：[1]Key Laboratory of Biometallurgy of Ministry of Education, Central South University [2]School of Resources Processing and Bioengineering, Central South University

出　　处：《Journal of Central South University》2009年第4期552-557,共6页中南大学学报（英文版）

基　　金：Projects(50621063, 50674101) supported by the National Natural Science Foundation of China

摘　　要：Twelve samples derived from different locations in south central area of China are treated by enrichment and spread-plate technique for initial screening. Seven chitinase-producing strains are isolated. The chitinase present in the culture supematant of strain CS-01 possesses the maximum activity of 0.118 U/mL. Analysis of the morphological feature and the ITS rDNA sequence reveals that strain CS-01 belongs to Aspergillus fumigatus. Production of the chitinase is regulated by a inducible way and the maximum activity appears at 36 h in colloidal chitin culture. Purification of the chitinase is carried out by salting out, gel filtrate chromatography and anion exchange chromatography sequentially. Native-PAGE and SDS-PAGE indicate that the chitinase from A. fumigatus CS-01 is a monomer with the relative molecular mass estimated to be 4.50 × 10^4. Its maximum activity appears at pH 5 and 55 ℃. The chitinase is stable at pH 4.0-7.5 and below 45 ℃.Twelve samples derived from different locations in south central area of China are treated by enrichment and spread-plate technique for initial screening. Seven chitinase-producing strains are isolated. The chitinase present in the culture supernatant of strain CS-01 possesses the maximum activity of 0.118 U/mL. Analysis of the morphological feature and the ITS rDNA sequence reveals that strain CS-01 belongs to Aspergillus fumigatus. Production of the chitinase is regulated by a inducible way and the maximum activity appears at 36 h in colloidal chitin culture. Purification of the chitinase is carried out by salting out, gel filtrate chromatography and anion exchange chromatography sequentially. Native-PAGE and SDS-PAGE indicate that the chitinase from A. fumigatus CS-01 is a monomer with the relative molecular mass estimated to be 4.50×104. Its maximum activity appears at pH 5 and 55 ℃ . The chitinase is stable at pH 4.0?7.5 and below 45 ℃.

关 键 词：ASPERGILLUSFUMIGATUS CHITINASE PURIFICATION 

分 类 号：Q814[生物学—生物工程]