Characterization of tricalcium phosphate solubilization by Stenotrophomonas maltophilia YC isolated from phosphate mines  

作　　者：肖春桥 池汝安 何环 张文学 

机构地区：[1]Key Laboratory of Biometallurgy of Ministry of Education, Central South University [2]Key Laboratory for Green Chemical Process of Ministry of Education, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology [3]Yunnan Phosphate Chemical Group Co. Ltd

出　　处：《Journal of Central South University》2009年第4期581-587,共7页中南大学学报（英文版）

基　　金：Project(2004CB619201) supported by the Major State Basic Research and Development Program of China;Project(Z200515002) supported by the Key Project Foundation of the Education Department of Hubei Province, China;Project(GCP200801) supported by the Open Research Fund of Key Laboratory for Green Chemical Process of Ministry of Education, China;Project(Q200811) supported by the Youths Science Foundation of Wuhan Institute of Technology, China

摘　　要：The phosphate solubilizing characteristics of a strain YC, which was isolated from phosphate mines (Hubei, China), were studied in National Botanical Research Institute’s phosphate (NBRIP) growth medium containing tricalcium phosphate (TCP) as sole phosphorus (P) source. The strain YC is identified as Stenotrophomonas maltophilia (S. maltophilia) based upon the results of morphologic, physiological and biochemical characteristics and 16S rRNA sequences analysis. The results show that the strain S. maltophilia YC can solubilize TCP and release soluble P in NBRIP growth medium. A positive correlation between concentration of soluble P and population of the isolate and a negative correlation between concentration of soluble P and pH in the culture medium are observed from statistical analysis results. Moreover, gluconic acid is detected in the culture medium by HPLC analysis. It indicates that the isolate can release gluconic acid during the solubilizing experiment, which causes acidification of the culture medium and then TCP solubilization. S. maltophilia YC has a maximal TCP solubilizing capability when using maltose as carbon source and ammonium nitrate as nitrogen source, respectively, in NBRIP growth medium.The phosphate solubilizing characteristics of a strain YC, which was isolated from phosphate mines （Hubei, China）, were studied in National Botanical Research Institute＇s phosphate （NBRIP） growth medium containing tricalcium phosphate （TCP） as sole phosphorus （P） source. The strain YC is identified as Stenotrophomonas maltophilia （S. maltophilia） based upon the results of morphologic, physiological and biochemical characteristics and 16S rRNA sequences analysis. The results show that the strain S. maltophilia YC can solubilize TCP and release soluble P in NBRIP growth medium. A positive correlation between concentration of soluble P and population of the isolate and a negative correlation between concentration of soluble P and pH in the culture medium are observed from statistical analysis results. Moreover, gluconic acid is detected in the culture medium by HPLC analysis. It indicates that the isolate can release gluconic acid during the solubilizing experiment, which causes acidification of the culture medium and then TCP solubilization. S. maltophilia YC has a maximal TCP solubilizing capability when using maltose as carbon source and ammonium nitrate as nitrogen source, respectively, in NBRIP growth medium.

关 键 词：tricalcium phosphate （TCP） Stenotrophomonas maltophilia （S. maltophilia） phosphate mines phosphorus （P） gluconic acid 

分 类 号：O611.3[理学—无机化学]