番木瓜pds基因时空表达的荧光定量PCR分析  

Temporal-spatial expression analysis of pds gene in papaya by real-time PCR

作　　者：高新征[1,2] 言普[2] 沈文涛[2] 周鹏[1,2] 

机构地区：[1]海南大学,海南海口570228 [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点实验室,海南海口571101

出　　处：《广西农业科学》2009年第6期610-613,共4页Guangxi Agricultural Sciences

基　　金：中央级事业单位基本科研业务费资助项目(2008)

摘　　要：以番木瓜黄果肉品种Dwarf solo和红果肉品种Sunrise solo为材料,利用实时荧光定量PCR技术,对2个番木瓜品种的不同组织器官以及果实发育成熟过程中5个时期的八氢番茄红素脱氢酶基因(pds)表达进行分析。结果表明,在2个番木瓜品种的果实、花、叶片中,pds在转录水平上均有表达,但不同组织部位pds表达量存在差异,在成熟果实中最高,其次为花、叶;在2个番木瓜品种的果实成熟过程中,pds的表达逐渐增加,以在红果中的增加变化更为明显,且pds在红果肉中的表达量高于黄果肉。因此,pds基因可能在调控番木瓜果肉颜色形成中起重要作用。今后将对番木瓜果实中psy、zds、lcy-e等基因的表达进行分析,以全面研究番木瓜类胡萝卜素合成及果肉颜色形成的机理。A yellow fruit papaya cuhivar Dwarf solo and a red fruit cultivar Sunrise solo were used as materials, and the pds gene expression in different organs and at five development stages of fruits was analyzed by SYBR Green I realtime qPCR. The results indicated that pds gene expressed in fruit, flower and leaf of two papaya cuhivars, but the expression level was different among the three organs. The expression of pds in mature fruit was much higher than those in flower and leaf, and that in flower was higher than that in leaf. The expression of pds increased step by step in the maturing process of fruit of two cultivars, and its change in red fruit was obvious. The expression level of pds was different between two cuhivars, and the expression of pds in mature fruit of Sunrise solo （red fruit） was higher than that in Dwarf solo （yellow fruit）. Therefore, pds gene may play an important role in regulating fruit color of papaya, and the expressions of psy, zds, and lcy-e genes in fruit of papaya would be analyzed to study the mechanism of carotenoid biosynthesis and fruit color formation in the future.

关 键 词：番木瓜 八氢番茄红素脱氢酶基因 基因表达 荧光定量PCR 

分 类 号：S667.9[农业科学—果树学]