分子标记辅助聚合抗褐飞虱基因选育杂交水稻抗性品种初步研究  

Preliminary study on breeding hybrid rice by pyramiding brown planthopper resistance genes through molecular marker-assisted selection

作　　者：孙荣科[1,2,3] 陈乔[4,2,3] 李孝琼[1,2,3] 陈英之[4,2,3] 韦绍丽[4,2,3] 阳海宁[1,2,3] 张月雄[5] 李容柏[2,3,5] 

机构地区：[1]广西大学生命科学与技术学院,南宁530005 [2]广西作物遗传改良生物技术重点开放实验室,南宁530007 [3]广西亚热带生物资源保护利用重点实验室,南宁530005 [4]广西大学农学院,南宁530005 [5]广西农业科学院水稻研究所,南宁530007

出　　处：《广西农业科学》2009年第6期654-657,共4页Guangxi Agricultural Sciences

基　　金：广西科学基金项目(桂科基0833025);国家"863"计划项目(2006AA10Z1C5);国家"973"前期基础研究专项项目(2007CB116305)

摘　　要：以携带有bph2和Bph3的水稻品系ME1为抗褐飞虱供体亲本,以保持系盟B、先B、天B,不育系RT300、RT181和恢复系R29等6个常用的杂交稻亲本为轮回受体亲本,通过杂交、回交和自交,并结合分子标记辅助选择,将bph2和Bph3基因导入到各杂交稻亲本中。结果6个回交组合在BC2F1代共获得13株双基因杂合株系;结合成型株农艺性状,选取其中6份的BC2F1代自交种子播种进行苗期抗虫鉴定,结果显示,各株系对褐飞虱均表现中抗水平;标记全部309个抗性植株的基因型,共获得21个聚合两个抗性基因的纯合株系。这些纯合材料将可用于组配抗褐飞虱杂交水稻。In this study, rice line ME1 contained two brown planthopper resistance genes bph2 and Bph3 was used as resistance donor to cross and baekcross with six receptor parents of hybrid rice including maintainer line Meng B, Xian B, Tian B, sterile line RT 300 and RT 181, and restorer line R 29, and genes bph2 and Bph3 were transferred to six receptor parents by molecular marker-assisted selection. The results showed that a total of 13 BC2F1 lines with bph2 and Bph3 heterozygous genotype was obtained from six backcross combinations. Combined with agronomic trait selection at the adult plant stage, six inbred seeds of BC2F1 were seeded to identify the BPH resistance at seedling stage. The results indicated that all of six BCEF2 lines had moderate resistance to BPH, the genotypes of 309 resistant plants were mapped and 21 homozygous strains with bph2 and Bph3 were successfully obtained. These homozygous materials would be useful in breeding hybrid rice resistance to BPH.

关 键 词：杂交水稻 稻褐飞虱 分子标记辅助选择 抗性育种 

分 类 号：S511.034[农业科学—作物学] S435.112.3