分子印迹基质固相分散-液相色谱法测定牛奶中的氯霉素残留  

Study on the determination of trace chloramphenicol residue in milk by HPLC with matrix solid-phase dispersion using molecularly imprinted polymer as an adsorbent

作　　者：王荣艳[1,2] 王培龙[1] 佘永新[1] 王静[1] 王锡昌[2] 钟耀广[2] 

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,北京100081 [2]上海海洋大学食品学院,上海200090

出　　处：《分析试验室》2009年第8期26-28,共3页Chinese Journal of Analysis Laboratory

基　　金："863"高新技术项目(2007AA10Z400);"十一五"国家科技支撑计划(2006BAD12B03-06)项目资助

摘　　要：以对模板分子具有较强识别特性的分子印迹聚合物为基质固相分散吸附剂,提取牛奶中痕量氯霉素,最后用HPLC法测定。研究了氯霉素分子印迹聚合物对样品中氯霉素的提取和净化效果,在优化条件下,方法的检出限为0.15 ng/mL,定量限为0.50 ng/mL。不同氯霉素添加量的回收率大于93.2%,RSD<5.9%。方法适用于牛奶中氯霉素残留的测定。The CAP was extracted by MSPD using molecularly imprinted polymer of CAP, the clean procedure was performed using HLB solid phase extraction cartridge and the quantitative analysis was carried out by HPLC. The clean effect of MSPD was examined. The results indicated that the limit of detection （LOD） and limit of quantity （LOQ） were 0.15 and 0.50 ng/mL respectively under optimum conditions. The developed method was applied to the determination of CAP in milk, the recoveries of different quantities of CAP are above 93.2 %, and the relative standard deviations （RSD） is below 5.9%. This method possesses the merits of convenient operation, high sensitivity, good repeatability, and no need for abundant solvents, which is a feasible method for analysis of CAP in milk samples.

关 键 词：氯霉素 分子印迹聚合物 基质固相分散 HPLC 牛奶 

分 类 号：O557.7[理学—热学与物质分子运动论]