流动注射化学发光法快速测定尿液中左亚叶酸钙  

Determination of calcium levofolinate in urine by flow injection chemiluminescence method

作　　者：王瑞琪[1] 黄玉明[2] 

机构地区：[1]重庆工商大学环境与生物工程学院,重庆400067 [2]西南大学化学化工学院,重庆400715

出　　处：《分析试验室》2009年第8期94-96,共3页Chinese Journal of Analysis Laboratory

摘　　要：在碱性介质中，左亚叶酸钙对鲁米诺-K3Fe（CN）6体系有显著的增敏作用，据此，建立了一种简单、快速测定左亚叶酸钙的流动注射化学发光新方法。在优化的实验条件下，该法测定左亚叶酸钙的线性范围为5．0×10^-8 -1．0×10^-5 g／mL；检出限（3σ）为2．0×10^-08 g／mL；对浓度为1．0×10^-06 g／mL的样品进行11次平行测定，相对标准偏差为1．3％。将此法用于尿液中左亚叶酸钙的测定，同时进行回收率实验。The redox reaction between luminol and potassium ferricyanide produces chemiluminescence （CL） in an alkaline medium. Based on the enhanced CL intensity of calcium levofolinate to above reaction, a new simple and rapid method for the determination of calcium levofolinate by chemiluminescence coupled with flow injection is proposed. Under the optimal conditions, the CL response is linear to calcium levofolinate concentration in the range of 5.0 × 10^-8 - 1.0 × 10^-5 g/mL with a detection limit of 2.0 × 10^-8 g/mL （3σ）. The relative standard deviation is 1.3% for 1.0 × 10^-6 g/mL calcium levofolinate （n = 11）. The proposed method was successfully applied to the analysis of calcium levofolinate in urine.

关 键 词：流动注射 化学发光 左亚叶酸钙 尿液 

分 类 号：O657.3[理学—分析化学]