Caspase3和iNOS在人挫伤脑组织中的时序性表达  

Time-order Expression of Caspase 3 and iNOS in Contused Human Brain Tissue

作　　者：蔡红星[1] 李周儒[1] 程言博[1] 董国凯[1] 仲靖芳[1] 殷文江[1] 

机构地区：[1]徐州医学院法医学教研室,江苏徐州221002

出　　处：《法医学杂志》2009年第4期241-245,共5页Journal of Forensic Medicine

基　　金：江苏省教育厅资助项目(04KJD320198)

摘　　要：目的研究人脑挫伤后不同时间半胱氨酸天冬氨酸蛋白酶3(caspase3)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)表达的变化,为法医实践中脑损伤时间的推断提供依据。方法选择30例因颅脑损伤死亡案例为损伤组,5例非颅脑损伤急死的案例为对照组,应用免疫组织化学和图像分析技术,分别于死亡后2h、4～8h、10～14h、1～2d、3～5d及8～11d检测脑组织标本中caspase3和iNOS的变化规律。结果经统计学分析:(1)caspase3阳性细胞表达在伤后2h以内组开始升高,与对照组相比差异有统计学意义(P<0.05),1～2d组表达强度增高,3～5d组仍见高表达(P<0.05),以后逐渐下降。(2)iNOS阳性细胞表达在2h以内组与对照组比较差异无统计学意义(P>0.05),伤后4～8h开始升高(P<0.05),1～2d组表达强度最高,以后逐渐下降,伤后8～11d仍有弱表达(P<0.05)。结论caspase3和iNOS的表达可能成为实际检案中脑损伤时间推断的有效依据。Objective To investigate the expression of caspase-3 and iNOS in different intervals and to provide evidence for estimation of injury intervals after brain contusion in human. Methods Thirty cases died of serious brain injury were included into the injury groups and 5 cases died of non-brain injury were served as control group. To analyze the changes of easpase-3 and iNOS expression in brain samples at different intervals （2 h, 4-8h, 10-14h, 1-2d, 3-5d, 8-11 d） by immunohistochemistry and auto-image analysis system. Results The level of caspase-3 expression started to increase in 2 hours after brain contusion compared to the control group（P〈0.05）. The level of caspase-3 expression continued to increase in 1-2 days and maintained high level in 3-5 days compared to the control group（P〈0.05）, then decreased gradually. There was no statistically significant difference between the expression level of iNOS in 2 hours with the control group（P〉0.05）. But the expression level of iNOS began to increase in 4-8 hours after brain contusion and reached its maximum in 1-2 days, then decreased. Weak expression of iNOS still could be detected in 8-11 days. Conclusion The expression of caspase-3 and iNOS can be used as effective evidence for human brain contusion interval.

关 键 词：法医病理学 脑损伤 半胱氨酸天冬氨酸蛋白酶3 诱导型一氧化氮合酶 损伤时间的推断 

分 类 号：DF795.1[医药卫生—法医学]