蛋白质界面取向的实验控制与表征  

Experimental Control and Characterization of Protein Orientation on Surfaces

作　　者：章爱娟[1] 谢韵[1] 周健[1] 

机构地区：[1]华南理工大学化学与化工学院广东省绿色化学产品技术重点实验室,广州510640

出　　处：《化学进展》2009年第7期1408-1417,共10页Progress in Chemistry

基　　金：国家自然科学基金项目(No.20706019;20876052);教育部新世纪优秀人才支持计划项目(No.NCET-07-0313);华南理工大学科研启动基金项目(No.B10-D6060010)资助

摘　　要：在生物材料、生物传感器、生物燃料电池等应用中都涉及到蛋白质界面吸附,而其中一个关键的科学问题就是蛋白质在基底界面上的吸附取向,控制好正确的取向是发挥蛋白质生物功能的关键。本文归纳和比较了各种用于控制蛋白质界面取向的固定化方法,总结了影响蛋白质界面吸附取向的因素,并对当前应用较多的表征蛋白质在界面吸附取向的方法进行了综述,最后指出蛋白质取向控制的机理研究将有助于推动相关应用领域的迅速发展。Controlling the orientation of proteins on surfaces plays a key role in keeping the function of adsorbed proteins, as it is very important for applications in biomaterials, biosensors, and bio-fuel ceils etc. In this paper, we review and compare different approaches to immobilize proteins with controlled protein orientation on surfaces, and discuss factors that influence protein orientation. Several experimental methods to characterize protein orientation on surfaces are also discussed. Breakthrough made in the understanding of the mechanism of protein orientation on surfaces will contribute to the development of related applications.

关 键 词：蛋白质吸附 取向 生物传感器 生物材料 分子模拟 

分 类 号：O647.32[理学—物理化学] O629.73[理学—化学]