卡巴胆碱对脓毒症大鼠肠组织缺血和氧自由基损伤的保护作用  

The protective effect of carbachol against intestinal ischemia and free radical injury in rats with sepsis

作　　者：胡森[1] 张立俭[1] 白慧颖[1] 包呈梅[1] 

机构地区：[1]解放军总医院第一附属医院烧伤研究所休克与多器官障碍实验室,北京100037

出　　处：《中国药物与临床》2009年第8期672-674,共3页Chinese Remedies & Clinics

基　　金：全军医学科研"十一五"专项课题(06Z055)

摘　　要：目的研究卡巴胆碱(CAR)对脓毒症大鼠肠组织缺血引起的脂质过氧化损伤的保护作用。方法雄性SD大鼠32只,采用盲肠结扎穿孔术(CLP)制备大鼠脓毒症模型。随机分为CLP组和卡巴胆碱干预组(CAR组),每组16只。CLP后立即静脉注射CAR 10μg/kg(CAR组)或等量生理盐水(CLP组)。用激光多普勒血流仪测定于CLP后6 h和12 h空肠黏膜血流量(IMBF);然后处死动物,取空肠组织,测定丙二醛(MDA)含量、黄嘌呤氧化酶(XOD)和二胺氧化酶(DAO)活性及组织含水率。结果CLP后6 h和12 h CAR组IMBF分别为(66±10)BFU和(56±9)BFU,显著高于CLP组[(46±10)BFU和(38±8)BFU](P<0.05)。CAR组空肠组织XOD活性和MDA含量显著低于CLP组,但DAO活性高于CLP组(P均<0.05)。CAR组空肠组织含水率也低于CLP组,6 h[(66.3±4.5)%vs(71.6±3.2)%]和12 h[(66.8±3.4)%vs(72.1±2.8)%]差异均有统计学意义(P均<0.05)。结论卡巴胆碱能改善脓毒症大鼠小肠缺血、抑制氧自由基生成,减轻肠组织水肿和功能损害。Objective To investigate the protective effect of carbachol （CAR） against intestinal ischemia and free radical injury in rats with sepsis. Methods Thirty-two Sprague-Dawley rats were projected to sepsis induced by cecal ligation and puncture （CLP）, and were divided randomly into two groups （n=16 each） the septic model group （CLP group） and CAR-treated group （CAR group）. Carbachol （10 μg/kg, CAR group） or saline （CLP group） was immediately injected via the penis vein. The blood flow of jejunum Mucosa （JMBF） was detected by a laser-Doppler flowmetry at 6 h and 12 h after CLP, respectively. The rats were then sacrificed, harvested for jejunum specimens, and evaluated for diamine oxidase （DAO）, xanthine oxidase （XOD）,malondialdehyde （MDA） and water content （dry to wet weight ratio） in the jejunum. Results The levels of JMBF of CAR group were （66±10） BFU and （56±9） BFU at 6 h and 12 h after CLP, both significantly higher than those of CLP group [（46±lO）BFU and （38±8）BFU] （P〈0.05）. The CAR group also showed significantly lower levels of XOD and MDA and higher level of DAO as compared with CLP group in jejunum （all P〈0.05）. Water contents of the jejunum in CAR group were obviously lower than those in CLP group at 6 h [（66.3±4.5）% vs.（71.6±3.2）%] and 12 h [（66.8±3.4）% vs.72.1±2.8）%] respectively （all P〈O.05 ）. Conclusion Carbachol showed protective effects by promoting visceral perfusion, inhibiting lipid oxidative production and alleviating visceral edema and dysfunction in rats with sepsis.

关 键 词：胆碱能激动剂 脓毒症 小肠 缺血 活性氧 

分 类 号：R965[医药卫生—药理学]