广东舟山眼镜蛇蛇毒镇痛活性成分的分离及其药理性质研究  

Isolation and Pharmacological Properties of Analgesic Fraction from Venom of Naja naja atra

作　　者：梁映霞[1,2] 韩丽萍[1] 江伟健[1] 赵树进[1] 

机构地区：[1]广州军区广州总医院,广东广州510010 [2]中山大学中山医学院,广东广州510080

出　　处：《中药材》2009年第7期1022-1025,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:研究广东舟山眼镜蛇蛇毒的镇痛活性成分,为广东舟山眼镜蛇蛇毒的开发利用,寻找新型镇痛药奠定基础。方法:采用对氨基苯甲酸-Sepharose 6B亲和色谱与Sephadex G-50凝胶色谱进行分离纯化;利用SDS-聚丙烯酰胺凝胶电泳法与高效液相色谱法进行纯度的鉴定;应用激光飞行质谱测定其相对分子质量;采用神经病理性疼痛模型评估产物的镇痛活性。结果:经过两步柱色谱法分离得到的产物为单一成分,高效液相色谱测定相对纯度达到95%;相对分子质量为6741.236 Da;在神经病理性疼痛模型,单次腹腔给药后镇痛作用显著,持续时间超过24 h。结论:广东舟山眼镜蛇毒经过两步柱色谱后得到一高纯度的镇痛活性产物,值得进一步开发研究。Objective： To separate main analgesic fraction from venom of Guangdong Naja naja atra, to establish the basis for the using of Naja naja atra and find new analgesic fraction. Methods ： Affinity chromatography and size exelusin were used to isolate the analgesic fraction from the venom of Naja naja atra, and then to determine its properties by biochemical methods, such as SDS-poly- acryamide gel electrophoresis （SDS-PAGE）, HPLC and Mole-toff. Results： HPLC showed its relative purity was 95% （HPLC）and Mw was 6741. 236 Da. We observed that peripheral administration of neurotoxin strongly reduced the mechanical allogynia and thermal hyperalgesia for 24 hours, associated with this neuropathy （L5 spinal nerve ligation）. Conclusion： The fraction from venom of Naja naja atra has significant analgesic effect and it is worth further developing.

关 键 词：眼镜蛇 分离纯化 理化性质 药理作用 

分 类 号：R996.3[医药卫生—毒理学]