益妇宁软胶囊对去卵巢大鼠生殖内分泌-免疫功能的影响  

Effects of Yi Fu Ning Soft Gelatin Capsules on Reproductive Endocrine-Immune Function of Ovariectomized Rats

作　　者：吴朝霞[1] 王晓华[2] 邓虹珠[3] 

机构地区：[1]新乡医学院生物化学与分子生物学教研室,河南新乡453003 [2]广州医学院生物化学与分子生物学教研室,广东广州510182 [3]南方医科大学中医系,广东广州510515

出　　处：《中药材》2009年第7期1083-1086,共4页Journal of Chinese Medicinal Materials

基　　金：广东省中医药局项目(400001)

摘　　要：目的:研究益妇宁软胶囊(YFN)对去卵巢大鼠生殖内分泌-免疫功能的影响。方法:3月龄SD雌性大鼠60只,50只行双侧卵巢切除术,随机分为YFN高剂量组(2.0 g/kg)、中剂量组(1.0 g/kg)、低剂量组(0.5 g/kg)、阳性对照组(己烯雌酚0.1 mg/kg)及模型对照组(model),每组10只,另10只为假手术组(sham)。术后4 w开始给药,给药12 w后用放射免疫法测定血清雌二醇(E2)、卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(Te)、孕激素(P)水平及白介素-2(IL-2)含量,用流式细胞术测定血T淋巴细胞CD亚群。结果:①与假手术组比较,模型组大鼠血清E2、Te、P水平显著下降(P<0.01),FSH、LH水平显著上升(P<0.05或P<0.01);血T淋巴细胞百分含量显著下降(P<0.05),CD4+T淋巴细胞亚群显著减少(P<0.01),CD4+/CD8+比值显著降低(P<0.01),CD8+T淋巴细胞亚群无显著性变化;血清白介素-2含量显著下降(P<0.01)。②益妇宁治疗后去卵巢大鼠血清E2、P水平显著上升(P<0.01),FSH、LH显著下降,血T淋巴细胞亚群CD3+、CD4+和CD4+/CD8+比值均显著升高,血清IL-2水平显著提高,高剂量组较中、低剂量组作用显著。结论:益妇宁软胶囊能升高去卵巢大鼠血清性激素水平,下调FSH、LH水平,改善失衡的T淋巴细胞亚群,促进IL-2的分泌,调节去卵巢大鼠紊乱的生殖内分泌-免疫网络。Objective ：To investigate the effect of Yi Fu Ning Soft Gelatin Capsules （YFN） on reproductive endocrine-immune function of ovariectomized rats. Methods ：60 3-month old female Sprague-Dawley rats were used,50 of them were ovariectomized and randomly divided into 5 groups ： ovariectomizy （OVX） group, OVX with diethylstilbestrol tablets （DT） group, OVX with YFN （ high dose, middle dose and low dose） group. The others were sham-operated group. The rats were administrated initially in the 4th week after the operation. After drugs had been given for 12 weeks the rats were sacrificed ,blood serum hormone, IL-2 content and T lymphocyte subpopulation were detected with methods radioimmunoassay and flow cytometry（FCM）. Results ： （1）Compared with sham group, the level of serum E2 ,Te and P significantly decreased（P 〈 0.01 ）, FSH, LH content significantly increased; Blood T lymphocyte subpopulation CD3^＋ ceils, CD4^ ＋ cells and CD4^＋ /CD8 ^＋ ratio significantly decreased, serum IL-2 content also significantly decreased （P 〈 0. 01 ）. （2） Compared with model group, after treated by YFN, the level of serum E2 and P significantly increased（P 〈 0. 01 ） , serum FSH and LH content significantly decreased ;T lymphocyte subpopulation CD3 ^＋ cells,CD4^＋ cells and CD4 ^＋/CD8 ^＋ ratio were improved significantly and serum interleukin-2 （IL-2） content increased significantly . Conclusion： YFN can increase serum sexual hormone content, reduce the level of FSH and LH, and improve imbalaneed T lymphocyte subpopulation, stimulate IL-2 excretion, which means YFN can regulate inordinate reproductive endocrine-immune network in ovariectomized rats.

关 键 词：益妇宁软胶囊 去卵巢大鼠 T淋巴细胞亚群 白介素-2 

分 类 号：R285.5[医药卫生—中药学]