荧光动力学法测定中药多糖抗羟自由基的活性  

Determination of the Eliminating Ratio of TCM Polysaccharide to Hydroxyl Radical by Kinetic Fluorescent Spectrophotometry

作　　者：冯慧萍[1] 莫云燕[1] 黄敏斯[1] 潘永强[1] 范华均[1] 

机构地区：[1]广东药学院分析化学教研室,广东广州510006

出　　处：《中药材》2009年第7期1087-1090,共4页Journal of Chinese Medicinal Materials

基　　金：广州市科技支撑计划资助项目(2008Z1-E301)

摘　　要：目的:探索羟自由基(.OH)与水杨酸氧化反应的动力学过程,建立荧光动力学法测定中药多糖抗.OH活性的方法。方法:应用荧光光度法探讨水杨酸、Fe2+及H2O2的浓度、pH以及温度对反应动力学曲线的影响,根据动力学曲线斜率的变化测定陈皮和灵芝中的多糖对.OH的清除率,并用维生素C进行方法重复性与可靠性的验证。结果:在室温λex=295 nm、λem=411 nm光谱条件下测得反应速率与水杨酸浓度的动力学方程为y=0.9818x-1.1801,r≈1。测定陈皮多糖对.OH的50%清除率(IC50)为78.01 mg/L,灵芝的IC50=232.5 mg/L,维生素C的IC50=24.52μg/L,RSD为0.23%(n=5)。结论:本方法灵敏可靠,可用于中药多糖成分抗.OH活性的测定。Objective：To explore reaction dynamic of hydroxyl radical（ · OH） and salicylic acid,and to determine the elimination ratios of TCM polysaccharide to · OH by kinetic fluorescent analysis. Methods ： The impact dynamics factors of this reaction were studied by fluorescent, such as the reaction of concentration, reaction time and temperature. The dynamical equation was built, a kinetic fluorescent spectrophotometry based on the reaction was developed to determine the elimination ratio. Repetitiveness and reliability of this method were tested by vitamin C. Results：The dynamical equation of reaction rate to salicylic acid was y = 0. 9818x - 1. 1801 under the condition of λ ex = 295 nm, λem = 411 nm at room temperature, r ≈ 1. The 50% elimination ratio（ IC50 ） of TCM polysaccharide of Tangerine peel and Ganoderma lucidum to · OH was 78.01 mg/L and 232. 5 mg/L, respectively. The IC50 of vitamin C was 24. 52 μg/L, RSD was 0. 23% （n = 5 ）. Conclusions：The method is sensitive and reliable, it can be used to determine the elimination ratio of TCM polysaccharide to · OH.

关 键 词：荧光动力学法 羟自由基 水杨酸 清除率 中药多糖 

分 类 号：R285.5[医药卫生—中药学]