杭白菊药液大孔树脂精制工艺研究  

Study on Refining Process of Flos Chrysanthemi Extract with Macroporous Resin

作　　者：于红宇 李艳卿 陈历雄 张军[2] 卫罡[2] 

机构地区：[1]广州市番禺区何贤纪念医院,广东广州511400 [2]广州中医药大学,广东广州510006

出　　处：《中药材》2009年第7期1125-1128,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:确定杭白菊药液大孔吸附树脂精制工艺的相关参数。方法:分别以绿原酸、木犀草素标定的总黄酮为指标,确定树脂种类、最大上样量、洗脱溶媒种类及用量,并以上述成分结合木犀草素的保留率为指标,验证大孔树脂工艺参数。结果:杭白菊药液在F型树脂的最大上样量以绿原酸计为10.8mg绿原酸/g干树脂,以木犀草素标定的总黄酮测得最佳洗脱溶媒为80%乙醇,洗脱溶媒用量为5倍柱体积;过柱后总黄酮、绿原酸、木犀草素精制度分别为589.2%、605.4%、650.1%。结果:杭白菊药液F型大孔吸附树脂精制工艺可保留极性较大的绿原酸和极性较小的总黄酮,达到"去粗存精"的纯化效果。Objective：To determine the parameters of refining process of Flos Chrysanthemi extract with macroporous adsorption resin. Methods： According to the chlorogenic acid and total fiavonoids, to investigate the kind of macoroporous resin, the maximum quantity of physic liquor, and the type and quantity of elution solvent. Then combining with the retention rate of luteolin, to verify the parameters of the process. Results：The maximum adsorption quantity of chlorogenic acid was 10. 8 mg/g dry resin,and the elution solvent was 5-fold column volume of 80% alcohol ; the purity of total flavonoids, chlorogenic acid and luteolin were 589.2% ,605.4%, 650. 1% ,respectively. Conclusion：The resin F can enrich the active components of Flos Chrysanthemi effectively.

关 键 词：杭白菊 总黄酮 绿原酸 木犀草素 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]