毛冬青注射液HPLC指纹图谱研究  

Study on Fingerprint of Maodongqing Injection by HPLC

作　　者：赖珊[1] 杨立伟[1] 欧国灯[1] 李泳雪 

机构地区：[1]广东省药品检验所,广东广州510180

出　　处：《中药材》2009年第7期1132-1134,共3页Journal of Chinese Medicinal Materials

基　　金：广东省中医药局建设中医药强省科研课题(1060035);广东省科技计划项目(2007B031407002)

摘　　要：目的:应用HPLC法建立毛冬青注射液的指纹图谱。方法:采用ODS-2 Hypersil C18(4.6 mm×200 mm,5μm)色谱柱,以乙腈和0.05%磷酸为流动相,梯度洗脱,流速1 mL/m in,柱温30℃,检测波长为325 nm。结果:建立了毛冬青注射液的指纹图谱,并检测了8批毛冬青注射液样品;采用"中药色谱指纹图谱相似度评价系统(2004A版)"标定了13个共有峰,相似度较高,各色谱峰分离较好,达到中药注射剂指纹图谱研究的技术要求。结论:本方法准确可靠,为更好地控制毛冬青注射液的内在质量提供了科学依据。Objective： To establish the fingerprint of Maodongqing injection by HPLC. Methods： The column of ODS-2 Hypersil Cls （4. 6 mm ×200 mm, 5 μm） was used. The mobile phase consisted of acetonitrile-0. 05% phosphoric acid with gradient elution. The flow rate was 1 mL/min, the column temperature was 30℃, and the detection wavelength was 325 nm. Results ： The fingerprint of Maodongqing injection was established, and 8 samples of different batches of Maodongqing injection were detected. Thirteen peaks in the chromatogram were marked by ＂Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine （Version 2004 A）＂. There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of the fingerprints of Chinese traditional medicine. Conclusion ： This method is accurate, reliable and provides a scientific basis for controlling the quality of Maodongqing injection.

关 键 词：毛冬青注射液 HPLC 指纹图谱 

分 类 号：R927.2[医药卫生—药学]