HPLC法测定湿热痹片中盐酸小檗碱的含量  

Determination of berberine hydrochlorde in Shirebi Tablets by HPLC

作　　者：金凤环 段彦杰[2] 

机构地区：[1]本溪市化学工业学校,辽宁本溪117000 [2]解放军总医院第二附属医院国际肿瘤治疗中心,北京100091

出　　处：《中国医药导报》2009年第23期52-54,共3页China Medical Herald

摘　　要：目的:建立测定湿热痹片中盐酸小檗碱含量的HPLC法。方法:采用高效液相色谱法对湿热痹片中的盐酸小檗碱进行含量测定,色谱柱为迪马Diamonsil(TM)钻石C18柱(4.6mm×250mm,5μm),流动相为乙腈-1%磷酸溶液(48∶52,每100毫升中加入十二烷基磺酸钠0.1g),流速为1.0ml/min,检测波长为265nm,柱温为25℃,进样量为10μl。结果:盐酸小檗碱在0.0186～0.3720μg范围内线性关系良好,r=0.9999;平均回收率为100.14%,RSD为1.35%。结论:本法操作简便、准确、重现性好,可用于湿热痹片的质量控制。Objective： To establish a HPLC method to determine berbefine hydrochlorde in Shirebi Tablets. Methods： The contents of berberine hydrochlorde were determined in Shirebi Tablets by HPLC. A Diamonsil （TM） Cls chromatographic column（4.6 mm×250 mm, 5μm） was used, the mobile phase was consisted of acetonitrile-0.1% phosphoric acid solution（g8：52） at the flow rate of 1.0 ml/min, the detection wavelength was 265 nm, the column temperature was 25℃ and the sample volume was 10 μl. Results： The linear range of berberine hydrochlorde was 0.018 6-0.372 0 μg （r=0.999 9）, the average recovery was 100.14% with a RSD of 1.35%. Conclusion： The method is easy to operate, and can provide accurate and reproducible results, thus is suitable for the quality control of Shirebi Tablets.

关 键 词：湿热痹片 盐酸小檗碱 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]