乙肝病毒血清标志物模型与HBV-DNA定量的相关性  

Relationship between model of serum markers of hepatitis B virus and HBV-DNA quantitative

作　　者：云天英[1] 李武莉[1] 陈韩[1] 钱士匀[1] 

机构地区：[1]海南医学院附属医院检验科,海南海口5701102

出　　处：《海南医学院学报》2009年第9期1165-1166,1173,共3页Journal of Hainan Medical University

基　　金：海南医学院科研基金资助学报项目(0020090173)~~

摘　　要：目的:探讨乙肝病毒血清标志物模型与HBV-DNA定量的相关性。方法:应用酶联免疫吸附试验(ELISA)和荧光定量聚合酶链反应技术(FQ-PCR),对865例乙型肝炎病毒携带者血清标本分别进行乙肝病毒血清标志物模型与HBV-DNA定量测定。结果:模式A(大三阳)的检出率最高,为96.1%,模式B(小三阳)的HBV-DNA检出率为79.0%,模式CHBV-DNA检出率为63.5%。结论:HbeAg是反映HBV复制活跃的可靠指标;血清HBeAg阴性,病毒并未停止复制;联合检测乙肝标志物和HBV-DNA水平有助于疾病的诊断、疗效提高及预后判断。Objective： To investigate the relationship between model of serum markers of hepatitis B virus and HBV-DNA quantitative. Methods： Eight sixty-five serum samples of subjects with hepatitis B virus were detected using Enzyme-linked immunosorbent assay （ELISA） and fluorescence quantitative polymerase chain reaction （FQ-PCR） for serum markers of hepatitis B virus in different models and quantitative determination of HBV-DNA respectively. Results： The detection rates of HBV-DNA in cases of positive model A, B, C were 96.1% , 79.0% and 63.5% respectively. Conclusion： HbeAg is a reliable indicator of vigorous replication of HBV, nega- tive HBeAg does not indicate the stop of the virus copying; combined detection of hepatitis B markers and HBV- DNA levels can enhance the accuracy of diagnosis, improve evaluation of therapy and prognosis.

关 键 词：乙肝病毒 血清标志物模型 HBV-DNA定量 荧光定量聚合酶链反应 

分 类 号：R512.62[医药卫生—内科学]