原发性胆汁性肝硬化患者抗SP100抗体与核点型抗核抗体的检测  

Detection of anti-SP100 and nuclear dots antibodies in patients with primary biliary cirrhosis

作　　者：刘妍[1] 闫惠平[1] 檀玉芬[1] 张海萍[1] 赵艳[1] 张欣[1] 冯霞[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染与免疫中心,北京100069

出　　处：《临床检验杂志》2009年第4期256-258,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金资助项目(30571723);北京市自然科学基金资助项目(7062033)

摘　　要：目的探讨抗SP100抗体与核点型抗核抗体对原发性胆汁性肝硬化(PBC)患者的临床意义。方法选取PBC患者100例,60例自身免疫性肝炎(AIH)患者、50例慢性乙型肝炎(HB)患者和50例慢性丙型肝炎(HC)患者作为疾病对照组,50例献血员(BD)作为健康人对照组。间接免疫荧光法(IFA)检测自身抗体,免疫印迹法检测抗SP100抗体。结果IFA法检测结果,抗核抗体(ANA)阳性57例,抗线粒体抗体(AMA)阳性100例,抗平滑肌抗体(SMA)阳性3例,细胞骨架抗体(CS)阳性5例。免疫印迹法检测PBC患者抗SP100阳性16例(阳性率16.0%),AIH患者抗SP100阳性4例(6.7%),HB、HC、BD对照均阴性。PBC患者抗SP100阳性组与阴性组ANA滴度比较,差异无统计学意义;抗SP100阳性组93.8%(15/16)表现核点型ANA,其次表现核点合并核膜型,而抗SP100阴性组主要表现核膜型(70.7%),其次为着丝点型、颗粒型等。PBC患者抗SP100阳性患者与阴性患者ALT、AST、TB il、GGT、ALP、IgG、IgA、IgM水平差异均无统计学意义(P>0.05)。结论抗SP100抗体阳性同时ANA为核点型有助于PBC患者的诊断。Abstract：Objective To study the significance of anti-SPIO0 and nuclear dots antibodies in diagnosing primary biliary cirrhosis （PBC）. Methods One hundred PBC patients,60 autoimmune hepatitis （AIH） ,50 chronic hepatitis B （HB） ,50 chronic hepatitis C （HC）, and 50 healthy blood donors were included. Autoantibodies were tested by immunofluorescent assay. Anti-SP100 was detected by Western blot analysis. Results Of 100 PBC patients, all were positive for AMA,57 for ANA,3 for SMA, and 5 for CS. Additionally, 16 PBC and 4 AIH patients were positive for anti-SP100,and no patient was positive for anti-SP100 in other groups. The ANA titer had no significant difference between anti-SP100 positive and negative patients;however, the ANA type was different. The most ANA type of anti-SP100 positive cases was nuclear dots （93.8%）, while the ANA type of anti-SP100 negative cases was nuclear membranes （70.7%）. The serum levels of ALT,AST,TBil,GGT,ALP,IgG,IgA,or IgM had no difference between anti-SPl00 positive and negative PBC patients （ P 〉 O. 05 ）. Conclusion The detection of SP100 and nuclear dots antibodies is helpful in diagnosing primary biliary cirrhosis.

关 键 词：原发性胆汁性肝硬化 抗核抗体 核点蛋白 

分 类 号：R446.6[医药卫生—诊断学]