交联琼脂糖凝胶表面修饰对其性状影响的研究  

Effect of surface modification on the characteristics of cross-linked agarose gel

作　　者：王巍[1] 徐文学[1] 任军[1] 谢健[1] 贾凌云[1] 

机构地区：[1]大连理工大学环境与生命学院,辽宁大连116023

出　　处：《应用化工》2009年第7期937-940,949,共5页Applied Chemical Industry

基　　金：国家高技术研究发展计划"863"项目(2008AA202Z210)

摘　　要：采用活化剂羰基二咪唑(CD I)活化琼脂糖凝胶并对其进行表面修饰,考察活化密度与CDI用量的关系和修饰前后凝胶耐压能力、粒径、外观形态、含水率等的变化。结果显示,琼脂糖表面70%的羟基可被CDI活化,最大活化密度可达4.4 mmol/g干胶,且在一定范围内活化密度与活化剂的加入量成正比。连接了不同分子的凝胶因为氮元素或芳环的引入导致凝胶内部以及凝胶与水分子所形成氢键的强度的变化,使其耐压能力有所提高,平均粒径增大,孔径普遍缩小,固形物的含量有所增加。且上述变化与修饰密度相关,较高的修饰密度下凝胶的各项参数都会发生较大改变。The cross-linked agarose gel was activated by carbonyldiimidazole （CDI） and modified surface. The relationship between the amount of CDI used and the activated density, as well as the e on the hanges on the gel＇ s anti-pressure ability, diameter, configuration and moisture content before and after the modification was examined. The results showed that 70% of the hydroxyl groups on the gel could be activated by CDI, the activated density could reach 4.4 mmol/g dry gel, and in a certain range the activated density was proportional to the amount of CDI. After connected with other molecules, the gel showed an improved anti-pressure ability, increased average diameter, smaller pores and increased solid percentage content, due to the weakening of hydrogen bond inside the beads and between the beads and water molecules caused by introduction of nitrogen or phenyt. All the changes mentioned above were related to the modification density, higher modification density resulted in greater changes in the characteristics of the gel.

关 键 词：交联琼脂糖凝胶 表面修饰 耐压性能 微观结构 含水率 

分 类 号：Q539.9[生物学—生物化学]