大豆异黄酮与脂肪、蛋白质含量基因定位分析  

QTL Mapping of Isoflavone,Oil and Protein Content in Soybean

作　　者：梁慧珍[1] 王树峰[1] 余永亮[1] 练云[1] 王庭峰[1] 位艳丽[1] 巩鹏涛[2] 刘学义[3] 方宣钧[2] 

机构地区：[1]河南省农业科学院经济作物研究所 国家大豆改良中心郑州分中心,郑州450002 [2]海南省热带农业资源开发利用研究所,海南三亚572025 [3]山西省农业科学院经济作物研究所,山西汾阳032200

出　　处：《中国农业科学》2009年第8期2652-2660,共9页Scientia Agricultura Sinica

基　　金：国家重点基础研究发展计划(“973”计划)项目(2008CB117005);农业科技成果转化资金(2008D00010209);国家转基因重大专项(2008ZX08004-005);“948”引进项目(2009-Z31)

摘　　要：【目的】研究大豆异黄酮与脂肪、蛋白质含量基因定位及相关性,为大豆品质改良、分子育种及基因克隆等应用提供理论依据。【方法】利用SSR技术,对晋豆23号和灰布支杂交构建的F13代大豆重组自交系群体的474个家系进行了连锁图谱的构建。在此基础上,利用WinQTLCart2.0软件分析了影响大豆异黄酮含量、脂肪含量和蛋白质含量3个重要品质性状的QTL,通过复合区间作图分析,检测QTL;同时,对异黄酮与脂肪、蛋白质的含量相关性分析。【结果】检测到23个QTL,其中控制异黄酮含量QTL有6个,分别定位在J、N、D2和G染色体的连锁群上;控制脂肪含量的QTL有11个,分别定位在第A1、A2、B2、C2和D2染色体的连锁群上;控制蛋白质含量的QTL有6个,分别定位在B2、C2、G和H1染色体的连锁群上。相关性分析结果表明:异黄酮与蛋白质含量呈极显著负相关;蛋白质和脂肪含量呈极显著负相关;蛋白质和蛋白质脂肪总量呈极显著正相关。【结论】3个重要品质性状的部分基因定位结果与其相关性分析是一致的,其结果对大豆品质育种应用有重要利用价值。[Objective] Dietary intake ofisoflavones has been shown to reduce the risk of several major diseases in humans. Therefore, breeding soybean [Glycine max （L.） Merrill] seeds with desirable isoflavone content would be beneficial to the food and healthy industries, but the environmental sensitivity of the trait complicates phenotypic selection. The objective of this study was to identify quantitative trait loci （QTL） and epistatic interactions associated with isoflavone contents, protein contents, oil contents in soybean seeds. [Method] An F13 recombinant inbred line（RIL） comprising 474 lines was derived from a cross between Jindou23 and Huibuzhi. SSR technique was employed for mapping the QTLs. [Result] The QTLs for isofiavone content, protein content, oil content were analyzed and 23 QTLs were detected based on the constructed linkage map. Six QTLs for isoflavone content were localized in linkage groups J, N, D2, and G; Eleven QTLs for oil content were localized in the linkage groups A1, A2, B2, C2 and D2 Six QTLs for protein content were localized in linkage groups B2, C2, G and HI. The correlation analysis demonstrated that the content of isoflavone was in significant correlation with protein content. Significantly negative correlations were existed between oil content and protein content, and significantly positive correlations were existed between protein content and the content of total protein and oil. [Conclusion] All these have laid an important basis for the marker assisted breeding of the soybean. According to analysis of this investigation, the phenotype correlations of quantitative traits may result from the correlations of QTL controlling those traits.

关 键 词：大豆 SSR QTL 品质 异黄酮 

分 类 号：S565.1[农业科学—作物学]