表皮生长因子与地塞米松对早产大鼠肺发育的影响  

Effects of Antenatal Epidermal Growth Factor and Dexamethasone on Lung Development in Premature Rats

作　　者：王爱红[1] 罗敏洁[2] 何红燕[3] 马桂霞[4] 史雪川[4] 林广裕[4] 马廉[4] 

机构地区：[1]南方医科大学附属南海医院儿科,广州528200 [2]汕头大学医学院 [3]汕头大学医学院第一附属医院骨科 [4]汕头大学医学院第二附属医院儿科,广东汕头515041

出　　处：《实用儿科临床杂志》2009年第14期1083-1084,1126,共3页Journal of Applied Clinical Pediatrics

基　　金：广东省科技厅项目资助(2005B30601022)

摘　　要：目的探讨表皮生长因子(EGF)和地塞米松(DEX)对早产大鼠呼吸窘迫综合征的防治作用。方法将72只胎鼠随机分为EGF、DEX2个干预组和9g/L盐水对照组,于孕19d剖腹取胎鼠,称取胎鼠体质量;通过光镜、图像分析观察胎鼠肺的形态结构;用免疫组织化学方法检测胎鼠肺组织表面活性蛋白A和B(SP-A及SP-B)的表达,并通过计算免疫组织化学平均灰度值比较各组SP-A及SP-B的表达。结果EGF组胎鼠体质量与对照组比较无明显差异[(1.39860±0.07305)g,(1.38630±0.03727)g],DEX组胎鼠体质量明显低于对照组[(1.31920±0.05326)g,(1.38630±0.03727)g]。光镜下EGF组与DEX组胎肺可见到明显的肺泡腔结构,对照组肺泡腔尚未发育;图像分析表明EGF组与DEX组胎肺的肺泡腔与肺泡间隔面积之比均大于对照组(Pa<0.01)。SP-A及SP-B在3组中均有表达,与对照组比较,SP-A及SP-B在EGF组和DEX组中的表达强度均显著增强(Pa<0.01)。结论EGF和DEX在SD大鼠妊娠第16-18天作用于母体,均能促进胎鼠肺的形态发育和表面活性物质的合成,对早产大鼠呼吸窘迫综合征的发生具有防治作用。二种药物对胎鼠肺的影响程度无明显不同,但产前应用DEX会降低胎鼠体质量,EGF无此不良反应。Objective To explore the preventive and therapeutic effects of antenatal epidermal growth factor（EGF） and Dexamethasone （DEX） on the premature rats with respiratory disease syndrome. Methods Seventy - two SD fetal rats were randomly divided into 3 groups ： control group, EGF group and DEX group. The body weight was measured. The histologic structure was observed with light microscope and light microscopic image system. Surfactant protein - A and B （SP -A and SP- B） antibody were determined by immunohistochemical staining respectively in each group,and were compared average gray scale value in each group, Resutlts The body weight of fetus in DEX group was smaller than that in control group [ （ 1. 398 60 ± 0. 073 05 ） g, （ 1. 386 30 ± 0. 037 27 ） g ] , but there was no apparent difference between EGF group and control group[ （1.319.20 ± 0. 053 26 ） g, （1. 386 30 ± 0. 037 27 ） g ]. Under light microscope, alveolar space could be more easily discerned in EGF group and DFX group than that in control group. The relative value of air spaces area and interalveolar septa area in EGF group and DEX group were significantly greater than that in control group（ P. 〈0.01 ）. The expression of SP- A and SP- B could be detected in each group. In contrast with control group, significant differences for SP - A and SP - B were expressed in EGF group and DEX group（ P 〈 0. 01 ）. Conclusions Prenatal maternal treatment with EGF and DEX on day 16 - 18 of gestation can promote the morphogenesis and increase the surfaetant levels of premature fetal lung. However, prenatal maternal treatment with DEX can decrease the body weight, EGF has no evident side effect on this respect.

关 键 词：表皮生长因子 地塞米松 胎肺 

分 类 号：R722.6[医药卫生—儿科]