滇型水稻细胞质雄性不育恢复基因Rf-D1(t)的克隆与遗传特性分析  

Cloning and Genetic Characterization of the Fertility Restorer Gene Rf-D1(t) for Dian-Type Cytoplasmic Male Sterility of Rice (Oryza sativa L.)

作　　者：朱建荣[1] 李东宣[1] 钱树琪[1] 李娟[1] 甘树仙[1] 周健[1] 乔德华[1] 陈丽娟[1] 

机构地区：[1]云南农业大学稻作研究所,昆明650201

出　　处：《分子植物育种》2009年第4期671-678,共8页Molecular Plant Breeding

基　　金：云南省应用基础研究重点项目(K2100295)资助

摘　　要：CMS/Rf测交F1结实率与花粉可育率检测表明,Ansanbyeo和南34对三种不同细胞质来源的粳稻不育系(CMS-DT1,CMS-BT和CMS-HL)都有较强的恢复力。根据已公布的水稻基因组序列,在水稻恢复基因定位区段内设计引物,确定出一对与两个滇型CMS的恢复系Ansanbyeo和南34恢复基因紧密连锁的分子标记引物M43804和M43558,首次克隆了滇型恢复基因Rf-D1(t),编码16个PPR蛋白。序列比对表明,该基因与BT型恢复基因Rf-1相似度达99%,两个恢复基因之间在ORF区仅存在一个碱基差异,即Rf-D1(t)第1149bp位的A变成Rf-1的C,并产生一个氨基酸的改编即K(赖氨酸)变成N(天冬酰胺)。该差异处于一个PPR结构域中,暗示该氨基酸是恢复基因的关键位点之一,它的改变可能影响对水稻CMS育性恢复力的变化。Testing the seed-setting and pollen fertility of hybrid FI, Ansanbyeo and Nan34 performed high restoration ability to different cytoplasmic male sterile lines （CMS-DT1, CMS-BT and CMS-HL）. According to the published rice genome sequence, primers were designed in the region office fertility restorer genes and a pair of molecular primers M43804 and M43558 which are closely linked to the restorer genes of two Dian-type CMS restorer lines Ansanbyeo and Nan34 was determined. Dian-type restorer gene Rf-D1 （t） was firstly cloned with code of 16 PPR proteins. Sequence comparison showed that this gene has 99% similarity to BT-type restorer gene Rf-1. There was only one base pair difference between the two restorer genes in the section of ORF, i.e. the A of Rf-D1 （t） changed into C of Rf-1 at 1 149 bp, and an amino acid code changing from K （lysine） to N （asparagines） was occurred. The difference is located in a PPR structure which means that amino acid might be one of the key loci of the restorer genes, and its alternation may affect the variation ofrestorability for rice CMS lines.

关 键 词：水稻 滇型CMS 恢复基因 PPR蛋白 

分 类 号：S511[农业科学—作物学]