中华稻蝗消化道内分泌细胞的鉴别与定位  

Identification and localization of endocrine cells in the digestive tract of Oxya chinensis(Orthoptera:Acrididae)

作　　者：陈洪洪[1] 潘洪珍[1] 张盛周[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000

出　　处：《昆虫学报》2009年第7期749-754,共6页Acta Entomologica Sinica

基　　金：安徽师范大学生物大分子进化重点实验室开放基金(2006B001);"动物生物学"安徽师范大学优秀学术团队基金;生物环境与生态安全省级重点实验室及安徽省重要生物资源保护与利用重点实验室建设基金(2007-10)

摘　　要：采用整块组织Grimelius银染法和过氧化物酶标记的链霉亲和素免疫组织化学技术,结合生物统计学分析,对中华稻蝗Oxyachinensis消化道内分泌细胞进行鉴别与定位。结果表明:嗜银细胞分布于中华稻蝗的胃盲囊、中肠和后肠各段,以中肠和直肠中最多(P<0.05),前肠中未见分布。免疫组织化学法检测出了五羟色胺(5-hydroxytryptamine,5-HT)、胃泌素(gastrin,Gas)、胰高血糖素(glucagon,Glu)和胰多肽(pancreatic polypeptide,PP)细胞,未检出生长抑素(somatostatin,SS)细胞。免疫阳性细胞分布于中肠和后肠中,前肠中未见分布。5-HT细胞和Gas细胞均主要分布于胃盲囊、中肠及直肠中,且均以直肠中最多(P<0.05)。Glu细胞在胃盲囊及整个中、后肠均有分布,在中肠和直肠中最多(P<0.05)。PP细胞主要分布于中肠、回肠和直肠中,中肠中分布密度最大(P<0.05)。本研究显示中华稻蝗消化道中存在多种内分泌细胞,它们的分布情况与其他节肢动物相比存在一定的共性,也有其一定的特异性,可能与中华稻蝗特定的消化道结构和消化生理功能有关。The endocrine cells in the digestive tract of Oxya chinensis were identified and localized by Grimelius silver impregnation and immunohistochemical method combining with biostatistical analysis. The results showed that argyrophil cells were detected in the gastric caecum, the midgut and throughout the hindgut, but not found in the foregnt by silver impregnation, with the highest density in the midgut and rectum （P 〈 0. 05 ）. 5- hydroxytryptaminc （5-HT）, gastrin （Gas）, glucagon （Glu）and pancreatic polypeptide （PP） cells were identified in gastric caecum, the midgut and the hindgnt by immunohistochemical method, but somatostatin （SS） cells were not detected throughout the digestive tract. Both 5-HT and Gas cells are distributed in the gastric caecum, midgut and rectum with the highest density in the rectum （P 〈 0. 05）. Glu cells are distributed throughout the midgut and the hindgut, with the highest density in the midgut and rectum （P 〈0.05）. PP cells are distributed in the midgut, ileum and rectum with the highest density in the midgut （P 〈0.05）. The results indicate that there are several types of endocrine cells in the digestive tract of O. chinensis ; and the distributional pattern of these cells are similar to those of other arthropods, but some unique features of the species were also found, which might be related to its special structure and physiological function of digestive tract.

关 键 词：中华稻蝗 消化道 嗜银细胞 内分泌细胞 免疫组织化学 

分 类 号：Q962[生物学—昆虫学]