光增白剂M2R对草地螟中肠围食膜的影响及对Bt毒力的增效作用  

Effect of fluorescent brightener 28(M2R) on peritrophic membrane of Loxostege sticticalis (Lepidoptera:Pyralidae) and its synergistic effect on Bt virulence

作　　者：尹姣[1] 郭巍[2] 李克斌[1] 曹雅忠[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 [2]河北农业大学河北省农作物病虫害生物防治工程技术中心,河北保定071001

出　　处：《昆虫学报》2009年第7期763-768,共6页Acta Entomologica Sinica

基　　金：国家重点基础研究发展规划项目(2006CB102007);国家科技支撑计划项目(2005BA529A03)

摘　　要：以光增白剂M2R作为影响因子,探讨其对草地螟Loxostege sticticalis幼虫围食膜(peritrophic membrane,PM)的作用机理。通过环境扫描电镜观察和生化测定研究了光增白剂对草地螟幼虫围食膜结构和蛋白质种类的影响,及其对Bt毒力的增效作用。结果表明:围食膜含有多种蛋白质,经SDS-PAGE测定至少有19条带,分子量在94kD以下,幼虫取食光增白剂可影响围食膜中几丁质结合蛋白(chitin binding proteins,CBPs)的含量。不同浓度的光增白剂可以对草地螟围食膜的形态结构产生明显的影响,正常的围食膜表面光滑致密、无孔洞和缝隙,增白剂处理的围食膜产生了孔缝。生测实验表明,添加光增白剂后能够显著缩短Bt的杀虫时间,降低Bt的使用浓度。可见,光增白剂可对草地螟围食膜产生损伤,进而提高了Bt的防治效果。Fluorescent brightener 28 is used as an influencing factor to study the mechanism of M2R on the peritrophic membrane （PM） of Loxostege sticticalis larvae. The environmental scanning electron microscopy and biochemical technique were employed to analyze the morphological structure and the protein profiles of the PM of L. sticticalis larvae treated by M2R, and the disruptive effect of M2R on the PM and its synergistic effect on Bt virulence were studied. The results indicated that there were many types of PM proteins. Separation of PM proteins by SDS-PAGE showed that there were at least 19 polypeptides with molecular weight less than 94 kD. The chitin binding proteins （CBP） content of PM could be influenced when the larvae were fed with 1.0% M2R. The surface of the normal PM was smooth and wrinkled without pores or slits while the surface of the treated PM had pores and slits. Although M2R did not affect the larval growth, it could enhance the sensitivity of larvae to Bt infection, shorten the killing time and reduce the application dose of Bt. These results suggest that the M2R could damage the PM of the larvae, and thus increase the control effect of Bt.

关 键 词：草地螟 光增白剂 围食膜 BT 增效作用 环境扫描电镜 

分 类 号：Q966[生物学—昆虫学]