自发性癫痫大鼠脑海马两种锌转运体表达的研究  

Expression of two zinc transporters in the hippocampus of spontaneously epileptic rats

作　　者：杨丹[1] 王清华[1] 孙凤[1] 王占友[2] 王玥[2] 高慧玲[2] 蔡际群[1] 

机构地区：[1]中国医科大学药学院药物毒理学教研室,沈阳110001 [2]中国医科大学药学院组胚教研室,沈阳110001

出　　处：《中华神经医学杂志》2009年第8期802-805,共4页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30270535）：教育部博士点基金（200601590030）

摘　　要：目的探讨反复癫痫发作后的自发性癫痫大鼠（SER）与正常Wistar大鼠脑海马锌转运体1和3的表达与癫痫的相关性，以及癫痫发作后脑海马内含锌神经元内锌稳态的改变情况。方法应用Westernblot和RT—PCR方法分析反复癫痫发作后的SER与正常Wistar大鼠脑海马锌转运体1和3表达情况。结果SER反复癫痫发作后脑海马锌转运体1蛋白及mRNA表达均明显高于正常Wistar大鼠，差异有统计学意义（P〈0．05）；锌转运体3蛋白表达与正常Wistar大鼠相比无明显改变，差异无统计学意义（P〉0．05）。结论反复癫痫发作后SER脑海马可能在神经元尤其突触后神经元内出现锌浓度增高现象，锌转运体1表达上调可通过降低神经元内Zn^2＋水平对抗癫痫发作引起的神经元损伤，从而起到保护神经元的作用。Objective To explore the correlation between hippocampal zinc transporter 1 and 3 expressions and epilepsy in spontaneously epileptic rats （ESRs） and normal rats, and observe the alternations of zinc homeostasis in zinc-containing neurons after seizure. Methods Western blot and RT-PCR were used to detect zinc transporter 1 and 3 expressions in the hippoeampus of both ESRs with recurrent seizures and normal Wistar rats. Results At both the mRNA and protein levels, hippocampal zinc transporter 1 expression in ESRs with recurrent seizures was significantly higher than that in normal Wistar rats, while hippocampal zinc transporter 3 protein expression was comparable between the two groups. Conclusion Hippocampal zinc transporter 1 expression is up-regnlated and Zn^2＋ in the hippoeampal neurons, especially the post-synaptic neurons, can be elevated in ESRs after recurrent seizure. Up-regulated zinc transporter 1 expression in the hippocampus may protect the neurons against seizure-induced injuries by decreasing Zn^2＋ levels in the neurons.

关 键 词：癫痫 锌 锌转运体 

分 类 号：R686[医药卫生—骨科学]