小麦糯性基因的多重PCR分子鉴定  

Molecular identification on Waxy genes in wheat using multiple-PCR

作　　者：卢龙斗[1] 侯彩玲[1] 陈龙[2] 殷贵鸿 邓传良[1] 高武军[1] 杨绪勤[1] 谭光轩[2] 

机构地区：[1]河南师范大学生命科学学院,新乡453007 [2]河南省周口师范学院生命科学系,周口466000 [3]河南省周口市农业科学院,周口466001

出　　处：《遗传》2009年第8期844-848,共5页Hereditas（Beijing)

基　　金：河南省重点科技攻关项目(编号:072102120011)资助

摘　　要：采用多重PCR的方法,对其反应条件进行优化,以获得用于小麦糯性(Wx)基因分析的稳定PCR体系。应用两对引物,分别扩增小麦Wx-A1、Wx-B1、Wx-D1基因,目的片段大小分别为:230bp/265bp、854bp和204bp。经反复验证,结果准确可靠,重复性好,成本低,可以在同一PCR反应体系中对3个Wx基因进行同时筛选鉴定。该体系可用于Wx蛋白基因的分子标记辅助选择,可以提高小麦淀粉品质评价和糯麦选育的效率。Multiple-PCR was conducted to establish a stable PCR system for identifying the three Wx genes in wheat. Two pairs of primers were employed to amplify Wx-A1, Wx-B1, and Wx-D1 genes of wheat, with the target sequences of 230 bp/265 bp, 854 bp, and 204 bp, respectively. The results showed that Wx-A1, Wx-B1, and Wx-D1 can be detected simultaneously in a single reaction. This method proved to be repeatable and low cost for evaluation of wheat quality properties in breeding program. This multiple-PCR technique can be efficiently used in marker-assisted selection for Wx genes, which will improve selection procedure for waxy wheat.

关 键 词：小麦 WX基因 多重PCR 分子标记辅助选择 

分 类 号：S512.1[农业科学—作物学]