卡巴胆碱对脓毒症大鼠肝氧自由基损伤的保护作用  

Protective effect of carbachol on liver free radical injury in rats with sepsis

作　　者：白慧颖[1] 张立俭[1] 包呈梅[1] 胡森[1] 冀文茹[1] 

机构地区：[1]解放军总医院第一附属医院烧伤研究所休克与多器官障碍实验室,北京100048

出　　处：《中国急救医学》2009年第8期681-683,共3页Chinese Journal of Critical Care Medicine

基　　金：全军医学科研“十一五”专项课题（No.067055）

摘　　要：目的研究卡巴胆碱（CAR）对脓毒症大鼠肝组织缺血引起的脂质过氧化损伤的保护作用。方法雄性SD大鼠32只，采用盲肠结扎穿孔术（CLP）制作大鼠脓毒症模型。随机分为CLP组、卡巴胆碱干预组（CAR组），每组16只。CLP术后立即静脉注射CAR10μg／ks（CAR组）或等量生理盐水（CLP组）。采用激光多普勒血流仪测定大鼠CLP术后6h和12h肝脏血流量；然后处死动物，取肝组织，测定丙二醛（MDA）含量、黄嘌呤氧化酶（XOD）和二胺氧化酶（DAO）活性及组织含水量。结果CLP术后6、12hCAR组肝脏血流量高于CLP组（P〈0．05）。CAR组肝脏XOD活性、MDA含量和ALT水平显著低于CLP组（P〈0．05），6h组、12h组差异均有统计学意义。CAR组肝脏组织含水量均低于CLP组（P〈0．05），6h组、12h组比较差异有统计学意义（P〈0．05）。结论卡巴胆碱能改善脓毒症大鼠肝缺血，抑制氧自由基生成，减轻肝组织水肿和功能损害。Objective To investigate protective effect of carbachol on on liver free radical injury in rats with sepsis. Methods Thiyty two Sprague - Dawley rats were projected to sepsis induced by cecal ligation and puncture（ CLP） , and divided randomly into two equal groups： septic model group（ CLP group） and septic model with CAR- treatment group（ CAR group）. Mter operation, CAR 10 μg/kg （CAR group） or saline （shame and CLP group） was immediately injected into penis vein. The blood flow of liver was detected by a laster - doppler flowmetry during the experiment. Animals were sacrificed at 6 hour and 12 hour after CLP respectively, and sepcimens of liver were harvested for evaluation of xanthine oxidase（ XOD ）, malondialdehyde（ MDA ） and assessment of the rate of water content （ratio of dry and wet weight ） of liver. Results The blood flow of liver of CAR group were significantly higher than those of CLP group both 6 h and 12 h,repectively （6 h,68. 92 ± 10. 45 VS 42. 61 ± 10. 37,P 〈 0. 05 ; 12 h,42.49 ± 9. 54 VS. 30. 12 ± 8.27 ,P 〈 0. 05 ）. It was showed signifant lower levels of XOD, MDA and ALT in CAR group than CLP group in liver both 6 h and 12 h, repeetively （ P 〈 0. 05 ）. The rates of tissue water contents of CAR group were obviously lower than those of CLP group in liver both 6 h and 12 h,repectively{ 6 h,70. 9 ±2.9 VS. 75.2 ±2. 2,P 〈0. 05;12 h,71.6 ±2. 7 VS. 74. 8 ±2. 2, P 〈0. 05）. Conclusion The results indicate that carbachal exert its protective effects by promoting liver peffusion, inhibiting lipid oxidative prouction and alleviate liver edema and dysfunction in rates with sepsis.

关 键 词：卡巴胆碱 脓毒症 肝 缺血 氧自由基 

分 类 号：R515.3[医药卫生—内科学]