四种方法对组培室空气消毒效果的研究  

Study on Air Disinfection of Tissue Culture Lab by Four Methods

作　　者：邹瑜[1] 林贵美[1] 韦华芳[1] 李朝生 牟海飞 

机构地区：[1]广西农业科学院生物技术研究所,广西南宁530007 [2]广西植物组培苗有限公司,广西南宁530007

出　　处：《北方园艺》2009年第8期117-119,共3页Northern Horticulture

基　　金：广西农业科学院科技发展基金资助项目(1999030);广西"新世纪十百千人才工程"专项资金资助项目(2006225号)

摘　　要：采用紫外线、空气灭菌器、艾叶＋苍术、艾叶4种方法，对组培实验室进行空气消毒试验，分析其灭菌效果。结果表明：空气灭菌器、0．5g／甜艾叶＋2．0g／耐苍术熏蒸消毒方法灭菌效果很好，灭菌率分别达82．7％和70．1％，达到了培养室含菌量低于50cfu／m^3的基本要求，在实际应用中，2种方法轮换使用效果最佳，组培污染率降低了50％～60％。使用中草药如艾叶、苍术等进行空气消毒灭菌，是植物组培生产中又一种新的空气灭菌方式。Four methods, ultraviolet radiation, air sterilizer, artemisia leaf, artemisia leaf with atractylodes were applied to sterilize the air of plant tissue culture lab. The result showed that two methods of air sterilizer, suffocating of 0. 5 g/m^3 artemisia leaf with 2. 0 g/m^3 atractylodes were preferable, and the rate of sterilize reach 82.7% and 70. 1 % respectively, fit for the standard of 50 cfu/m^3 bacterium and fungi content of culture lab. The two methods can be applied in turn for sterilize of culture lab, and reduce the pollution rate by 50%-60%. Application of artemisia leaf and atractylodes could be utilized for air sterilize in culture lab.

关 键 词：组培苗 空气消毒 污染 苍术 艾叶 

分 类 号：Q94-338[生物学—植物学]