尖锐湿疣患者外周血Foxp3^＋CD4^＋CD25^＋调节性T细胞的检测及意义  

Detection of Foxp3^＋CD4^＋CD25^＋ regulatory T cells in the peripheral blood of patients with condyloma acuminatum and its significance

作　　者：徐妍[1] 叶俊[1] 朱可建[1] 程浩[1] 朱宁 陈贤祯[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院皮肤科,杭州310016

出　　处：《中华传染病杂志》2009年第8期484-486,共3页Chinese Journal of Infectious Diseases

基　　金：浙江省自然科学基金资助项目（2080199）

摘　　要：目的了解尖锐湿疣患者外周血Foxp3^＋CD4^＋CD25^＋调节性T细胞的表达水平，探讨其在尖锐湿疣发生发展机制中的作用。方法分别收集30例尖锐湿疣患者（复发15例，初发15例）及20例健康者外周抗凝静脉血，分离出外周血单个核细胞。藻红蛋白（PE）标记抗CD4单抗，异硫氰酸荧光素（FITC）标记抗CD25单抗，细胞破膜后PE标记的抗Foxp3单抗行细胞内染色，三色流式细胞术分析Foxp3^＋CD4^＋CD25^＋调节性T细胞比例。组间比较采用ANOVA检验。结果外周血中Foxp3^＋CD4^＋CD25^＋调节性T细胞水平在尖锐湿疣患者组为（3．4±1．0）％，在复发组为（4．7±1．2）％，均显著高于健康对照组的（1．2±0．5）％（P〈0．01）。外周血Foxp3^＋CD4^＋CD25^＋调节性T细胞水平在初发组为（2．1±1．0）％，高于健康对照组，但差异无统计学意义，而在复发组明显高于初发组（P〈0．05）。结论尖锐湿疣患者外周血Foxp3^＋CD4^＋CD25^＋调节性T细胞数量增加，这种细胞免疫功能失调可能与其免疫学发病机制有关。Objective To observe the levels of Foxp3^＋CD4^＋CD25^＋ regulatory T cells in the peripheral blood of condyloma acuminatum （CA） patients and investigate their roles in the pathogenesis of CA. Methods The peripheral blood was collected from 30 CA patients （including 15 with relapsing and 15 with first onset） and 20 healthy controls. Peripheral blood mononuclear cells （PBMC） were isolated and stained with anti-human CD4-PE-Cy5 and anti-human CD25-fluorescein isothiocyanate （FITC） monoclonal antibodies on cell membrane, followed by intracellular staining with anti human Foxp3 PE. The percentage of Foxp3^＋CD4^＋CD25^＋ regulatory T cells was detected by three-color flow eytometry. Comparison between groups was done by ANOVA test. Results The percentages of Foxp3^＋CD4^＋CD25^＋ regulatory T cells among total CD4＋ T cells in CA patients and relapsing CA group were （3.4 ± 1.0） % and （4.7 ± 1.2）%, respectively, which were both significantly higher than that in healthy control group [（1. 2±0. 5）%, P〈0. 01]. Furthermore, that in first onset CA group was （2.1 ±1.0）%, which was higher than that in healthy control group, but without statistical significance; but that in relapsing CA group was significantly higher than that in first onset group （P〈0.05）. Conclusions The number of Foxp3^＋CD4^＋CD25^＋ regulatory T cells increases in the peripheral blood of CA patients. The disorder of cellular immunity may be involved in the immunopathogenesis of CA.

关 键 词：尖锐湿疣 抗原 CD4 T淋巴细胞 受体 白细胞介素 转录因子 免疫 细胞 

分 类 号：R686[医药卫生—骨科学]