欧文氏菌T85-166果胶酶基因克隆与序列分析  

Gene Cloning and Sequence Analysis on Pectinase of Erwinia T85-166

作　　者：李琦[1] 戴小阳[1] 刘正初[1,2] 谢达平[1] 王文芳[1] 宋娟[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]农业部茎纤维生物质与工程微生物重点开放实验室,湖南长沙410205

出　　处：《湖南农业科学》2009年第7期8-10,共3页Hunan Agricultural Sciences

基　　金：国家"863"计划资助课题(2006AA02Z155)

摘　　要：根据果胶酶Pel基因在欧文氏菌基因组上的分布特点,通过合理设计引物,从欧文氏菌株T85-166克隆出3个果胶酶Pel基因(Pel-1、Pel-2和Pel-3),它们分别编码372、373和374个氨基酸。序列分析表明,Pel-1前105及后100个氨基酸序列与Pel-2完全相同,但与Pel-3有较大差异,而中间170个左右的氨基酸序列与Pel-3则高度相似。初步构建了3个Pel基因的工程菌。According to the distributing feature of pectinase Pel gene in the genome of Erwinia, two special primers were designed to clone pectinase Pel gene from Erwinia T85-166.Three pectinase Pel gene （Pel-1, Pel-2 and Pel-3） were cloned successfully and coding 372, 373, 374 amino acids （AA）respectively. The results of sequence analysis indicated that the sequences of forward 105 and last 100 AA in Pel-1 and Pel-2 were the same, but had a significant different with Pel-3; the sequences of middle 170 AA in Pel-1 and Pel-3 were identical. Three engineering strains of Pel gene were preliminary constructed.

关 键 词：果胶酶 果胶酸裂解酶 欧文氏菌 克隆 PCR 

分 类 号：S188.41[农业科学—农业基础科学]