杜仲毛状根培养条件优化及其桃叶珊瑚苷药用成分的研究  

Hairy root culture optimization and aucubin medical composition production of Eucommia ulmoides

作　　者：杨蕊[1] 张家辉[1] 孙雪梅[1] 孙敏[1] 

机构地区：[1]西南大学生命科学学院三峡库区生态环境教育部重点试验室,重庆400715

出　　处：《中国中药杂志》2009年第15期1902-1905,共4页China Journal of Chinese Materia Medica

摘　　要：目的:诱导杜仲毛状根的发生及筛选优良毛状根系。方法:利用发根农杆菌C58C1诱导杜仲不同外植体产生毛状根,并利用HPLC测定桃叶珊瑚苷含量。结果:采用杜仲无菌苗胚轴经菌液浸染20min,共培养3d可得到最佳转化效果。液体培养的生长量优于固体培养,最有利于毛状根生物量和药用成分的积累,并筛选到桃叶珊瑚苷高产根系E1。结论:可通过毛状根的培养来获得桃叶珊瑚苷药用成分。Objective： To introduce the hairy root of Eucommia ulmoides, and screen put the excellent roots. Method： Hairy rootswere obtained by infecting the different explants of E. ulmoides. Aucubin content was detected by HPLC. Result： The best transformation condition was embryonic axis infected by twenty minutes bacillus water dip-dye and three-day s co-culture. Liquid culture was better than solid culture, and advantageous to accumulating of biomass and medical contents of hairy root. The El was the excellent roots. Conclusion ： Hairy root of E. ulmoides will be useful for the production of active components in aucubin.

关 键 词：杜仲 毛状根 桃叶珊瑚苷 

分 类 号：R282[医药卫生—中药学]