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作 者:张村[1] 肖永庆[1] 李丽[1] 李文[1] 殷小杰[1]
出 处:《中国中药杂志》2009年第15期1949-1951,共3页China Journal of Chinese Materia Medica
基 金:国家自然科学基金项目(30672666)
摘 要:目的:对栀子果实、果仁、果皮中环烯醚萜苷类成分进行比较研究。方法:HPLC法同时测定2个主要成分(geni-pin gentiobioside,geniposide)含量,Kromasil C18柱,流动相为乙腈-0.3%甲酸水溶液12:88,检测波长238nm,流速1.0mL.min-1,柱温35℃。结果:4个成分在测定范围内线性关系较好(r>0.9994),平均回收率分别为99.11%,98.47%。栀子果实不同部位中均含有这2种环烯醚萜苷类成分,且含量有较大差异。结论:2种成分以栀子果仁中含量最高,为揭示栀子果仁、果皮的不同临床功效提供了实验依据。Objective: To compare the contents of iridoid among the fruit, seed, and pericarp from Gardenia jasminoides. Method: The contents of geniposide and genipin gentiobioside were determined simultaneously by HPLC. A Kromasil C18 column at 35肖永庆 was used with the acetonitrile-0. 3% formic acid anhydrous ( 12:88 ) as the mobile phases. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL · min ^-1. Result : The obtained linearity of the two components was better over 0. 999 4 and the average recoveries were 99. 11% , 98.47% , respectively. The results exhibited the remarkable variation among the different parts of the fruit. Conclusion: The contents of the two constituents in seed were higher than in pericarp.
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