复合膜异位诱导成骨研究  

Heterotopic complex membrane induces bone formation

作　　者：林崇韬[1] 李红艳[1] 闫嘉晴[1] 

机构地区：[1]吉林大学口腔医院牙周病科,吉林长春130041

出　　处：《华西口腔医学杂志》2009年第4期357-360,共4页West China Journal of Stomatology

基　　金：吉林省科技厅自然科学基金资助项目(20020658)

摘　　要：目的将基因重组人骨形态发生蛋白-2(rhBMP-2)与胶原、聚乳酸/聚羟基乙酸共聚体(PLGA)复合,制成rhBMP-2/Co/PLGA复合膜,观察其异位诱导成骨情况,评价其生物特性。方法取雄性昆明小鼠48只,随机分成Co/PLGA复合膜组与rhBMP-2/Co/PLGA复合膜组。在小鼠右后肢大腿肌间隙内分别植入2种复合膜材料。术后7、14、28d,各组分别处死8只动物,标本行肉眼、软X线照相和组织学观察。结果2组动物术后情况良好。Co/PLGA复合膜组术后7d复合膜开始溶解,14d膜结构不连续,28d大部分溶解;软X线和组织学观察未见异位骨生成。rhBMP-2/Co/PLGA复合膜组软X线和组织学观察均有骨样结构形成,与Co/PLGA复合膜组相比复合膜降解速度缓慢。结论Co/PLGA是rhBMP-2的良好载体,参与rhBMP-2诱导成骨活性的表达,使其在局部发挥更持久的生物活性。Objective The composite membrane was made by recombinant human bone morphogenetic protein-2 （rhBMP-2）, collagen （Co）, polylactic-co-glyeolic acid （PLGA）. To observe the ectopic bone formation and evaluate the capability of rhBMP-2/Co/PLGA complex membrane. Methods 48 male Kunming adult mice were divided into two groups randomly： Co/PLGA complex membrane group and rhBMP-2/Co/PLGA complex membrane group. After etherized, an incision was made on the outer muscle and two groups of complex membranes were implanted into the fight side hind limb muscle pouch. 8 mice from each group were sacrificed at 7, 14, 28 d. The hind limbs were removed, examined by soft X-ray, HE staining and light microscope examination. Results Wounds healed well, no infections and rejections were observed. In the Co/PLGA group, partial implanted membrane degradated and absorbed at 7 d. The membrane collagen fiber appeared loose at 14 d. The membrane lost its intact to disrupted at 28 d. No ectopic bone formation was found. In the rhBMP-2/Co/PLGA group, ectopic bone formation was found by eye, soft X- ray and histological examinations. The rhBMP-2/Co/PLGA complex membrane degraded slower than Co/PLGA complex membrane. Conclusion Co/PLGA can provide a carrier for rhBMP-2 to have stronger effect of biological activity.

关 键 词：胶原 聚乳酸/聚羟基乙酸共聚体 重组人骨形态发生蛋白-2 

分 类 号：R318[医药卫生—生物医学工程]